Tat28-35SL8-specific CD8+ T lymphocytes are more effective than Gag181-189CM9-specific CD8+ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay

John T. Loffredo; Eva G. Rakasz; Juan Pablo Giraldo; Sean P. Spencer; Kelly K. Grafton; Sarah R. Martin; Gnankang Napoé; Levi J. Yant; Nancy A. Wilson; David I. Watkins

doi:10.1128/JVI.79.23.14986-14991.2005

Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay

John T. Loffredo, Eva G. Rakasz, Juan Pablo Giraldo, Sean P. Spencer, Kelly K. Grafton, Sarah R. Martin, Gnankang Napoé, Levi J. Yant, Nancy A. Wilson, David I. Watkins

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Epitope-specific CD8⁺ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8 ⁺ T lymphocytes. Certain epitope-specific CD8⁺ T lymphocytes may be better than others at suppressing viral replication. We compared the antiviral efficacy of two immunodominant CD8⁺ T lymphocyte responses-Tat_28-35SL8 and Gag_181-189CM9-by using a functional in vitro assay. Viral suppression by Tat-specific CD8 ⁺ T lymphocytes was consistently greater than that of Gag-specific CD8⁺ T lymphocytes. Such differences in antigen-specific CD8 ⁺-T-Iymphocyte efficacy may be important for selecting CD8 ⁺ T lymphocyte epitopes for inclusion in future HIV vaccines.

Original language	English (US)
Pages (from-to)	14986-14991
Number of pages	6
Journal	Journal of virology
Volume	79
Issue number	23
DOIs	https://doi.org/10.1128/JVI.79.23.14986-14991.2005
State	Published - Dec 2005
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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Loffredo, J. T., Rakasz, E. G., Giraldo, J. P., Spencer, S. P., Grafton, K. K., Martin, S. R., Napoé, G., Yant, L. J., Wilson, N. A., & Watkins, D. I. (2005). Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay. Journal of virology, 79(23), 14986-14991. https://doi.org/10.1128/JVI.79.23.14986-14991.2005

Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay. / Loffredo, John T.; Rakasz, Eva G.; Giraldo, Juan Pablo; Spencer, Sean P.; Grafton, Kelly K.; Martin, Sarah R.; Napoé, Gnankang; Yant, Levi J.; Wilson, Nancy A.; Watkins, David I.

In: Journal of virology, Vol. 79, No. 23, 12.2005, p. 14986-14991.

Research output: Contribution to journal › Article › peer-review

Loffredo, JT, Rakasz, EG, Giraldo, JP, Spencer, SP, Grafton, KK, Martin, SR, Napoé, G, Yant, LJ, Wilson, NA & Watkins, DI 2005, 'Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay', Journal of virology, vol. 79, no. 23, pp. 14986-14991. https://doi.org/10.1128/JVI.79.23.14986-14991.2005

Loffredo JT, Rakasz EG, Giraldo JP, Spencer SP, Grafton KK, Martin SR et al. Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay. Journal of virology. 2005 Dec;79(23):14986-14991. https://doi.org/10.1128/JVI.79.23.14986-14991.2005

Loffredo, John T. ; Rakasz, Eva G. ; Giraldo, Juan Pablo ; Spencer, Sean P. ; Grafton, Kelly K. ; Martin, Sarah R. ; Napoé, Gnankang ; Yant, Levi J. ; Wilson, Nancy A. ; Watkins, David I. / Tat_28-35SL8-specific CD8⁺ T lymphocytes are more effective than Gag_181-189CM9-specific CD8⁺ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay. In: Journal of virology. 2005 ; Vol. 79, No. 23. pp. 14986-14991.

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abstract = "Epitope-specific CD8+ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8 + T lymphocytes. Certain epitope-specific CD8+ T lymphocytes may be better than others at suppressing viral replication. We compared the antiviral efficacy of two immunodominant CD8+ T lymphocyte responses-Tat28-35SL8 and Gag181-189CM9-by using a functional in vitro assay. Viral suppression by Tat-specific CD8 + T lymphocytes was consistently greater than that of Gag-specific CD8+ T lymphocytes. Such differences in antigen-specific CD8 +-T-Iymphocyte efficacy may be important for selecting CD8 + T lymphocyte epitopes for inclusion in future HIV vaccines.",

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