Tastants evoke cAMP signal in taste buds that is independent of calcium signaling

Kristina R. Trubey, Schartess Culpepper, Yutaka Maruyama, Sue C. Kinnamon, Nirupa Chaudhari

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We previously showed that rat taste buds express several adenylyl cyclases (ACs) of which only AC8 is known to be stimulated by Ca2+. Here we demonstrate by direct measurements of cAMP levels that AC activity in taste buds is stimulated by treatments that elevate intracellular Ca2+. Specifically, 5 μM thapsigargin or 3 μM A-23187 (calcium ionophore), both of which increase intracellular Ca2+ concentration ([Ca 2+]i), lead to a significant elevation of cAMP levels. This calcium stimulation of AC activity requires extracellular Ca2+, suggesting that it is dependent on Ca2+ entry rather than release from stores. With immunofluorescence microscopy, we show that the calcium-stimulated AC8 is principally expressed in taste cells that also express phospholipase Cβ2 (i.e., cells that elevate [Ca2+]i in response to sweet, bitter, or umami stimuli). Taste transduction for sucrose is known to result in an elevation of both cAMP and calcium in taste buds. Thus we tested whether the cAMP increase in response to sucrose is a downstream consequence of calcium elevation. Even under conditions of depletion of stored and extracellular calcium, the cAMP response to sucrose stimulation persists in taste cells. The cAMP signal in response to monosodium glutamate stimulation is similarly unperturbed by calcium depletion. Our results suggest that tastant-evoked cAMP signals are not simply a secondary consequence of calcium modulation. Instead, cAMP and released Ca2+ may represent independent second messenger signals downstream of taste receptors.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume291
Issue number2
DOIs
StatePublished - Aug 4 2006

Fingerprint

Taste Buds
Calcium Signaling
Calcium
Adenylyl Cyclases
Sucrose
Sodium Glutamate
Thapsigargin
Calcium Ionophores
Phospholipases
Calcimycin
Second Messenger Systems
Fluorescence Microscopy
Rats
Microscopic examination
Modulation

Keywords

  • Calcium-sensitive adenylyl cyclase
  • Capacitative entry
  • Cross talk
  • Taste transduction

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Tastants evoke cAMP signal in taste buds that is independent of calcium signaling. / Trubey, Kristina R.; Culpepper, Schartess; Maruyama, Yutaka; Kinnamon, Sue C.; Chaudhari, Nirupa.

In: American Journal of Physiology - Cell Physiology, Vol. 291, No. 2, 04.08.2006.

Research output: Contribution to journalArticle

Trubey, Kristina R. ; Culpepper, Schartess ; Maruyama, Yutaka ; Kinnamon, Sue C. ; Chaudhari, Nirupa. / Tastants evoke cAMP signal in taste buds that is independent of calcium signaling. In: American Journal of Physiology - Cell Physiology. 2006 ; Vol. 291, No. 2.
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