Targets of gene amplification and overexpression at 17q in gastric cancer

Asta Varis, Maija Wolf, Outi Monni, Marja Leena Vakkari, Arto Kokkola, Chris Moskaluk, Henry Frierson, Steven M. Powell, Sakari Knuutila, Anne Kallioniemi, Wa’el El-Rifai

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141 Scopus citations

Abstract

DNA copy number gains and amplifications at 17q are frequent in gastric cancer, yet systematic analyses of the 17q amplicon have not been performed. In this study, we carried out a comprehensive analysis of copy number and expression levels of 636 chromosome 17-specific genes in gastric cancer by using a custom-made chromosome 17-specific cDNA microarray. Analysis of DNA copy number changes by comparative genomic hybridization on cDNA microarray revealed increased copy numbers of 11 known genes (ERBB2, TOP2A, GRB7, ACLY, P1P5K2B, MPRL45, MKP-L, LHX1, MLN51, MLN64, and RPL27) and seven expressed sequence tags (ESTs) that mapped to 17q12-q21 region. To investigate the genes transcribed at the 17q, we performed gene expression analyses on an identical cDNA microarray. Our expression analysis showed overexpression of 8 genes (ERBB2, TOP2A, GRB2, AOC3, AP2B1, KRT14, JUP, and ITGA3) and two ESTs. Of the commonly amplified transcripts, an uncharacterized EST AA552509 and the TOP2A gene were most frequently overexpressed in 82% of the samples. Additional studies will be initiated to understand the possible biological and clinical significance of these genes in gastric cancer development and progression.

Original languageEnglish (US)
Pages (from-to)2625-2629
Number of pages5
JournalCancer Research
Volume62
Issue number9
StatePublished - May 1 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Varis, A., Wolf, M., Monni, O., Vakkari, M. L., Kokkola, A., Moskaluk, C., Frierson, H., Powell, S. M., Knuutila, S., Kallioniemi, A., & El-Rifai, W. (2002). Targets of gene amplification and overexpression at 17q in gastric cancer. Cancer Research, 62(9), 2625-2629.