Abstract
A set of electrostatically charged, fluorescent, and superparamagnetic nanoprobes was developed for targeting cancer cells without using any molecular biomarkers. The surface electrostatic properties of the established cancer cell lines and primary normal cells were characterized by using these nanoprobes with various electrostatic signs and amplitudes. All twenty two randomly selected cancer cell lines of different organs, but not normal control cells, bound specifically to the positively charged nanoprobes. The relative surface charges of cancer cells could be quantified by the percentage of cells captured magnetically. The activities of glucose metabolism had a profound impact on the surface charge level of cancer cells. The data indicate that an elevated glycolysis in the cancer cells led to a higher level secretion of lactate. The secreted lactate anions are known to remove the positive ions, leaving behind the negative changes on the cell surfaces. This unique metabolic behavior is responsible for generating negative cancer surface charges in a perpetuating fashion. The metabolically active cancer cells are shown to a unique surface electrostatic pattern that can be used for recovering cancer cells from the circulating blood and other solutions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1887-1898 |
| Number of pages | 12 |
| Journal | Theranostics |
| Volume | 6 |
| Issue number | 11 |
| DOIs | |
| State | Published - 2016 |
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Keywords
- Biomarker
- Cell metabolism
- Glycolysis
- Lactate secretion
- Nanoprobe
- Surface charge
- Targeting
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Cite this
Targeting negative surface charges of cancer cells by multifunctional nanoprobes. / Chen, Bingdi; Le, Wenjun; Wang, Yilong; Li, Zhuoquan; Wang, Dong; Ren, Lei; Lin, Ling; Cui, Shaobin; Hu, Jennifer; Hu, Yihui; Yang, Pengyuan; Ewing, Rodney C.; Shi, Donglu; Cui, Zheng.
In: Theranostics, Vol. 6, No. 11, 2016, p. 1887-1898.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeting negative surface charges of cancer cells by multifunctional nanoprobes
AU - Chen, Bingdi
AU - Le, Wenjun
AU - Wang, Yilong
AU - Li, Zhuoquan
AU - Wang, Dong
AU - Ren, Lei
AU - Lin, Ling
AU - Cui, Shaobin
AU - Hu, Jennifer
AU - Hu, Yihui
AU - Yang, Pengyuan
AU - Ewing, Rodney C.
AU - Shi, Donglu
AU - Cui, Zheng
PY - 2016
Y1 - 2016
N2 - A set of electrostatically charged, fluorescent, and superparamagnetic nanoprobes was developed for targeting cancer cells without using any molecular biomarkers. The surface electrostatic properties of the established cancer cell lines and primary normal cells were characterized by using these nanoprobes with various electrostatic signs and amplitudes. All twenty two randomly selected cancer cell lines of different organs, but not normal control cells, bound specifically to the positively charged nanoprobes. The relative surface charges of cancer cells could be quantified by the percentage of cells captured magnetically. The activities of glucose metabolism had a profound impact on the surface charge level of cancer cells. The data indicate that an elevated glycolysis in the cancer cells led to a higher level secretion of lactate. The secreted lactate anions are known to remove the positive ions, leaving behind the negative changes on the cell surfaces. This unique metabolic behavior is responsible for generating negative cancer surface charges in a perpetuating fashion. The metabolically active cancer cells are shown to a unique surface electrostatic pattern that can be used for recovering cancer cells from the circulating blood and other solutions.
AB - A set of electrostatically charged, fluorescent, and superparamagnetic nanoprobes was developed for targeting cancer cells without using any molecular biomarkers. The surface electrostatic properties of the established cancer cell lines and primary normal cells were characterized by using these nanoprobes with various electrostatic signs and amplitudes. All twenty two randomly selected cancer cell lines of different organs, but not normal control cells, bound specifically to the positively charged nanoprobes. The relative surface charges of cancer cells could be quantified by the percentage of cells captured magnetically. The activities of glucose metabolism had a profound impact on the surface charge level of cancer cells. The data indicate that an elevated glycolysis in the cancer cells led to a higher level secretion of lactate. The secreted lactate anions are known to remove the positive ions, leaving behind the negative changes on the cell surfaces. This unique metabolic behavior is responsible for generating negative cancer surface charges in a perpetuating fashion. The metabolically active cancer cells are shown to a unique surface electrostatic pattern that can be used for recovering cancer cells from the circulating blood and other solutions.
KW - Biomarker
KW - Cell metabolism
KW - Glycolysis
KW - Lactate secretion
KW - Nanoprobe
KW - Surface charge
KW - Targeting
UR - http://www.scopus.com/inward/record.url?scp=84995403898&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84995403898&partnerID=8YFLogxK
U2 - 10.7150/thno.16358
DO - 10.7150/thno.16358
M3 - Article
C2 - 27570558
AN - SCOPUS:84995403898
VL - 6
SP - 1887
EP - 1898
JO - Theranostics
JF - Theranostics
SN - 1838-7640
IS - 11
ER -