Targeted neuronal lesion induced by photosensitizing dyes

L. T. Wang-Bennett, D. J. Liebl, G. N. Bennett

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Free radical-induced phototoxicity mediated by laser irradiation was investigated in the rabbit facial nerve. Azure-C, mesoporphyrin, or the dye conjugated to the protein carrier horseradish peroxidase were injected into the levator alae nasi muscle. Two to 7 days after uptake and laser exposure, nerve sections showed varying degrees of cellular modifications including: severe membrane degradation and associated lipid peroxide granules, distended mitochondria, and mitochondrial loss. Immunoblots of homogenates from treated nerves revealed specific changes in neurofilament and myelin basic protein. The site specific damage produced in vivo by photosensitizing dye resembles abnormalities in aging neurons and in Batten's disease, both hypothesized to be cases of free radical-peroxidation reactions. These reactions differ from those found in transection and crush lesions.

Original languageEnglish (US)
Pages (from-to)122-128
Number of pages7
JournalBrain Research
Volume534
Issue number1-2
DOIs
StatePublished - Nov 26 1990
Externally publishedYes

Keywords

  • Axonal transport
  • Facial nerve
  • Free radical
  • Laser
  • Nerve degeneration
  • Photosensitizing dye
  • Phototoxicity

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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