Targeted neuronal lesion induced by photosensitizing dyes

L. T. Wang-Bennett; D. J. Liebl; G. N. Bennett

doi:10.1016/0006-8993(90)90121-Q

Targeted neuronal lesion induced by photosensitizing dyes

L. T. Wang-Bennett, D. J. Liebl, G. N. Bennett

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Free radical-induced phototoxicity mediated by laser irradiation was investigated in the rabbit facial nerve. Azure-C, mesoporphyrin, or the dye conjugated to the protein carrier horseradish peroxidase were injected into the levator alae nasi muscle. Two to 7 days after uptake and laser exposure, nerve sections showed varying degrees of cellular modifications including: severe membrane degradation and associated lipid peroxide granules, distended mitochondria, and mitochondrial loss. Immunoblots of homogenates from treated nerves revealed specific changes in neurofilament and myelin basic protein. The site specific damage produced in vivo by photosensitizing dye resembles abnormalities in aging neurons and in Batten's disease, both hypothesized to be cases of free radical-peroxidation reactions. These reactions differ from those found in transection and crush lesions.

Original language	English (US)
Pages (from-to)	122-128
Number of pages	7
Journal	Brain research
Volume	534
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(90)90121-Q
State	Published - Nov 26 1990
Externally published	Yes

Keywords

Axonal transport
Facial nerve
Free radical
Laser
Nerve degeneration
Photosensitizing dye
Phototoxicity

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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10.1016/0006-8993(90)90121-Q

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@article{a87f989e91ac47e89f1e0a7cab7405e1,

title = "Targeted neuronal lesion induced by photosensitizing dyes",

abstract = "Free radical-induced phototoxicity mediated by laser irradiation was investigated in the rabbit facial nerve. Azure-C, mesoporphyrin, or the dye conjugated to the protein carrier horseradish peroxidase were injected into the levator alae nasi muscle. Two to 7 days after uptake and laser exposure, nerve sections showed varying degrees of cellular modifications including: severe membrane degradation and associated lipid peroxide granules, distended mitochondria, and mitochondrial loss. Immunoblots of homogenates from treated nerves revealed specific changes in neurofilament and myelin basic protein. The site specific damage produced in vivo by photosensitizing dye resembles abnormalities in aging neurons and in Batten's disease, both hypothesized to be cases of free radical-peroxidation reactions. These reactions differ from those found in transection and crush lesions.",

keywords = "Axonal transport, Facial nerve, Free radical, Laser, Nerve degeneration, Photosensitizing dye, Phototoxicity",

author = "Wang-Bennett, {L. T.} and Liebl, {D. J.} and Bennett, {G. N.}",

note = "Funding Information: Acknowledgement. This material is based in part upon work supported by the Texas Advanced Technology Program under Grant 1165. We appreciate Dr. Cesar D. Fermin's comment on the manuscript.",

year = "1990",

month = nov,

day = "26",

doi = "10.1016/0006-8993(90)90121-Q",

language = "English (US)",

volume = "534",

pages = "122--128",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Targeted neuronal lesion induced by photosensitizing dyes

AU - Wang-Bennett, L. T.

AU - Liebl, D. J.

AU - Bennett, G. N.

N1 - Funding Information: Acknowledgement. This material is based in part upon work supported by the Texas Advanced Technology Program under Grant 1165. We appreciate Dr. Cesar D. Fermin's comment on the manuscript.

PY - 1990/11/26

Y1 - 1990/11/26

N2 - Free radical-induced phototoxicity mediated by laser irradiation was investigated in the rabbit facial nerve. Azure-C, mesoporphyrin, or the dye conjugated to the protein carrier horseradish peroxidase were injected into the levator alae nasi muscle. Two to 7 days after uptake and laser exposure, nerve sections showed varying degrees of cellular modifications including: severe membrane degradation and associated lipid peroxide granules, distended mitochondria, and mitochondrial loss. Immunoblots of homogenates from treated nerves revealed specific changes in neurofilament and myelin basic protein. The site specific damage produced in vivo by photosensitizing dye resembles abnormalities in aging neurons and in Batten's disease, both hypothesized to be cases of free radical-peroxidation reactions. These reactions differ from those found in transection and crush lesions.

AB - Free radical-induced phototoxicity mediated by laser irradiation was investigated in the rabbit facial nerve. Azure-C, mesoporphyrin, or the dye conjugated to the protein carrier horseradish peroxidase were injected into the levator alae nasi muscle. Two to 7 days after uptake and laser exposure, nerve sections showed varying degrees of cellular modifications including: severe membrane degradation and associated lipid peroxide granules, distended mitochondria, and mitochondrial loss. Immunoblots of homogenates from treated nerves revealed specific changes in neurofilament and myelin basic protein. The site specific damage produced in vivo by photosensitizing dye resembles abnormalities in aging neurons and in Batten's disease, both hypothesized to be cases of free radical-peroxidation reactions. These reactions differ from those found in transection and crush lesions.

KW - Axonal transport

KW - Facial nerve

KW - Free radical

KW - Laser

KW - Nerve degeneration

KW - Photosensitizing dye

KW - Phototoxicity

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U2 - 10.1016/0006-8993(90)90121-Q

DO - 10.1016/0006-8993(90)90121-Q

M3 - Article

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AN - SCOPUS:0025201140

VL - 534

SP - 122

EP - 128

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -

Targeted neuronal lesion induced by photosensitizing dyes

Abstract

Keywords

ASJC Scopus subject areas

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