Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion

Bipin C. Dash, T. A. Harikrishnan, Ritu Goila, Shweta Shahi, Hoshang Unwalla, Sajid Husain, Akhil C. Banerjea

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long truncated HIV-1 Env transcript by DNA enzyme 6339. Protein independent and Mg2+ dependent specific cleavage products were obtained. As soon as 5 min after mixing equimolar concentrations of DNA enzyme and substrate RNA, more than 50% cleavage was observed which increased steadily over a period of 4 h. Very little cleavage was obtained at 1 mM MgCl2 concentration which improved significantly when the concentration of MgCl2 was increased up to 20 mM. Specific inhibition of cell membrane fusion caused by the interaction of gp160 and CD4 in HeLa cells was observed when the above DNA enzyme was used. Thus, these chemically synthesized DNA enzymes could prove to be very useful for in vivo application.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalFEBS Letters
Volume431
Issue number3
DOIs
StatePublished - Jul 24 1998

Fingerprint

Cell Fusion
HIV-1
Fusion reactions
Genes
Magnesium Chloride
DNA
Enzymes
Membrane Fusion
HeLa Cells
Antiviral Agents
Cell membranes
Cell Membrane
RNA
Substrates
Proteins

Keywords

  • Cell membrane fusion
  • DNA enzyme
  • HIV-1 Env gene
  • Syncytia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Dash, B. C., Harikrishnan, T. A., Goila, R., Shahi, S., Unwalla, H., Husain, S., & Banerjea, A. C. (1998). Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion. FEBS Letters, 431(3), 395-399. https://doi.org/10.1016/S0014-5793(98)00799-6

Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion. / Dash, Bipin C.; Harikrishnan, T. A.; Goila, Ritu; Shahi, Shweta; Unwalla, Hoshang; Husain, Sajid; Banerjea, Akhil C.

In: FEBS Letters, Vol. 431, No. 3, 24.07.1998, p. 395-399.

Research output: Contribution to journalArticle

Dash, BC, Harikrishnan, TA, Goila, R, Shahi, S, Unwalla, H, Husain, S & Banerjea, AC 1998, 'Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion', FEBS Letters, vol. 431, no. 3, pp. 395-399. https://doi.org/10.1016/S0014-5793(98)00799-6
Dash, Bipin C. ; Harikrishnan, T. A. ; Goila, Ritu ; Shahi, Shweta ; Unwalla, Hoshang ; Husain, Sajid ; Banerjea, Akhil C. / Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion. In: FEBS Letters. 1998 ; Vol. 431, No. 3. pp. 395-399.
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