Target contact regulates expression of synaptotagmin genes in spinal motor neurons in vivo

Jason A. Campagna, David Prevette, Ronald W. Oppenheim, John Bixby

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

During neuromuscular development, neuronal contact with peripheral targets is associated with an increase in synaptic vesicle protein (SVP) gene expression, suggesting that target contact and upregulation of SVP genes are causally related. To test this idea, we analyzed the developmental expression pattern of synaptotagmin (syt) mRNAs in the chick lateral motor column (LMC) using in situ hybridization. Syt I mRNA in the LMC is upregulated from Embryonic Day 4.5 (E4.5) to E5.5, coincident with the time these neurons begin to make contact with their muscle targets. In contrast, levels of mRNA for neurofilament do not change during this time. Extirpation of the limb bud prior to motor axon outgrowth eliminates the increase in syt I mRNA ipsilaterally. Later in development, there is a switch in syt isoform abundance in the LMC, with syt II mRNA being upregulated between E15 and E20 and syt I mRNA being downregulated. Our results suggest that contact with targets upregulates syt I gene expression during neuromuscular synapse formation in vivo, and that a later stage of synaptic maturation involves changes in SVP isoform abundance.

Original languageEnglish
Pages (from-to)377-388
Number of pages12
JournalMolecular and Cellular Neurosciences
Volume8
Issue number6
DOIs
StatePublished - Dec 1 1996

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Synaptotagmins
Motor Neurons
Synaptotagmin I
Gene Expression
Messenger RNA
Synaptic Vesicles
Synaptotagmin II
Protein Isoforms
Up-Regulation
Limb Buds
Intermediate Filaments
Synapses
In Situ Hybridization
Proteins
Down-Regulation
Neurons
Muscles

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Target contact regulates expression of synaptotagmin genes in spinal motor neurons in vivo. / Campagna, Jason A.; Prevette, David; Oppenheim, Ronald W.; Bixby, John.

In: Molecular and Cellular Neurosciences, Vol. 8, No. 6, 01.12.1996, p. 377-388.

Research output: Contribution to journalArticle

Campagna, Jason A. ; Prevette, David ; Oppenheim, Ronald W. ; Bixby, John. / Target contact regulates expression of synaptotagmin genes in spinal motor neurons in vivo. In: Molecular and Cellular Neurosciences. 1996 ; Vol. 8, No. 6. pp. 377-388.
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