Tandem action of exercise training and food restriction completely preserves ischemic preconditioning in the aging heart

P. Abete, G. Testa, G. Galizia, F. Mazzella, David Della Morte, D. De Santis, C. Calabrese, F. Cacciatore, G. Gargiulo, N. Ferrara, G. Rengo, V. Sica, C. Napoli, F. Rengo

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Ischemic preconditioning (IP) has been proposed as an endogenous form of protection against ischemia reperfusion injury. IP, however, does not prevent post-ischemic dysfunction in the aging heart but may be partially corrected by exercise training and food restriction. We investigated the role of exercise training combined with food restriction on restoring IP in the aging heart. Effects of IP against ischemia-reperfusion injury in isolated hearts from adult (A, 6 months old), sedentary 'ad libitum' fed (SL), trained ad libitum fed (TL), sedentary food-restricted (SR), trained- and food-restricted senescent rats (TR) (24 months old) were investigated. Norepinephrine release in coronary effluent was determined by high performance liquid cromatography. IP significantly improved final recovery of percent developed pressure in hearts from A (p<0.01) but not in those from SL (p=NS) vs unconditioned controls. Developed pressure recovery was partial in hearts from TL and SR (64.3 and 67.3%, respectively; p<0.05 vs controls) but it was total in those from TR (82.3%, p=NS vs A; p<0.05 vs hearts from TL and SR). Similarly, IP determined a similar increase of norepinephrine release in A (p<0.001) and in TR (p<0.001, p=NS vs adult). IP was abolished by depletion of myocardial norepinephrine stores by reserpine in all groups. Thus, IP reduces post-ischemic dysfunction in A but not in SL. Moreover, IP was preserved partially in TR and SR and totally in TR. Complete IP maybe due to full restoration of norepinephrine release in response to IP stimulus.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalExperimental Gerontology
Volume40
Issue number1-2
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Aging of materials
Exercise
Food
Norepinephrine
Recovery
Reperfusion Injury
Reserpine
Restoration
Rats
Effluents
Pressure
Liquids

Keywords

  • Aging
  • Caloric restriction
  • Exercise training
  • Ischemic preconditioning

ASJC Scopus subject areas

  • Aging
  • Medicine(all)
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

Cite this

Tandem action of exercise training and food restriction completely preserves ischemic preconditioning in the aging heart. / Abete, P.; Testa, G.; Galizia, G.; Mazzella, F.; Della Morte, David; De Santis, D.; Calabrese, C.; Cacciatore, F.; Gargiulo, G.; Ferrara, N.; Rengo, G.; Sica, V.; Napoli, C.; Rengo, F.

In: Experimental Gerontology, Vol. 40, No. 1-2, 01.01.2005, p. 43-50.

Research output: Contribution to journalArticle

Abete, P, Testa, G, Galizia, G, Mazzella, F, Della Morte, D, De Santis, D, Calabrese, C, Cacciatore, F, Gargiulo, G, Ferrara, N, Rengo, G, Sica, V, Napoli, C & Rengo, F 2005, 'Tandem action of exercise training and food restriction completely preserves ischemic preconditioning in the aging heart', Experimental Gerontology, vol. 40, no. 1-2, pp. 43-50. https://doi.org/10.1016/j.exger.2004.10.005
Abete, P. ; Testa, G. ; Galizia, G. ; Mazzella, F. ; Della Morte, David ; De Santis, D. ; Calabrese, C. ; Cacciatore, F. ; Gargiulo, G. ; Ferrara, N. ; Rengo, G. ; Sica, V. ; Napoli, C. ; Rengo, F. / Tandem action of exercise training and food restriction completely preserves ischemic preconditioning in the aging heart. In: Experimental Gerontology. 2005 ; Vol. 40, No. 1-2. pp. 43-50.
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AU - Testa, G.

AU - Galizia, G.

AU - Mazzella, F.

AU - Della Morte, David

AU - De Santis, D.

AU - Calabrese, C.

AU - Cacciatore, F.

AU - Gargiulo, G.

AU - Ferrara, N.

AU - Rengo, G.

AU - Sica, V.

AU - Napoli, C.

AU - Rengo, F.

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N2 - Ischemic preconditioning (IP) has been proposed as an endogenous form of protection against ischemia reperfusion injury. IP, however, does not prevent post-ischemic dysfunction in the aging heart but may be partially corrected by exercise training and food restriction. We investigated the role of exercise training combined with food restriction on restoring IP in the aging heart. Effects of IP against ischemia-reperfusion injury in isolated hearts from adult (A, 6 months old), sedentary 'ad libitum' fed (SL), trained ad libitum fed (TL), sedentary food-restricted (SR), trained- and food-restricted senescent rats (TR) (24 months old) were investigated. Norepinephrine release in coronary effluent was determined by high performance liquid cromatography. IP significantly improved final recovery of percent developed pressure in hearts from A (p<0.01) but not in those from SL (p=NS) vs unconditioned controls. Developed pressure recovery was partial in hearts from TL and SR (64.3 and 67.3%, respectively; p<0.05 vs controls) but it was total in those from TR (82.3%, p=NS vs A; p<0.05 vs hearts from TL and SR). Similarly, IP determined a similar increase of norepinephrine release in A (p<0.001) and in TR (p<0.001, p=NS vs adult). IP was abolished by depletion of myocardial norepinephrine stores by reserpine in all groups. Thus, IP reduces post-ischemic dysfunction in A but not in SL. Moreover, IP was preserved partially in TR and SR and totally in TR. Complete IP maybe due to full restoration of norepinephrine release in response to IP stimulus.

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