T lymphocytes can recognize determinants unique to neuropeptides of guinea pig myelin basic protein containing a single D-isomer amino acid substitution

Robert B Levy, S. Miller, N. M. Garcia, M. Jones, G. H. Fisher, E. H. Man

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present studies were undertaken to examine how the substitution of racemized forms of selected amino acids in synthetic peptides of guinea pig myelin basic protein (GPMBP) would alter the host's immunological ability to recognize such molecules. Using peptides from the 69-84 sequence of GPMBP containing a D-serine at position 70 or 75 (69-84[D-ser70 or D-ser75]) or D-aspartate at position 82 (69-84[D-asp82]), the findings demonstrated that the position of the diastereomer substitution on these neuropeptides was critical with respect to the ability of the immune system to recognize the molecule. Thus substitution of D-asp at position 82 or D-ser at position 75 abrogated the abilities of these peptides to induce experimental autoimmune encephalitis and proliferation of host T cells. In contrast, a peptide containing a D-ser70 residue was capable of inducing clinical disease in rats, as well as stimulating T lymphocytes from 69-84(D-ser70)-injected animals. Moreover, although this D-peptide was shown to share at least some determinant(s) with the 69-84 peptide, the use of 69-84(D-ser70)-stimulated cell lines demonstrated that some epitope(s) unique to this molecule could stimulate CD4+ syngeneic T cells.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalJournal of Neuroscience Research
Volume25
Issue number1
StatePublished - Jan 1 1990

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Myelin Basic Protein
Amino Acid Substitution
Neuropeptides
Guinea Pigs
T-Lymphocytes
Peptides
D-Aspartic Acid
Serine
Epitopes
Immune System
Amino Acids
Cell Line
interleukin-2 (59-72)

Keywords

  • Autoimmune
  • Brain
  • Racemization
  • T cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

T lymphocytes can recognize determinants unique to neuropeptides of guinea pig myelin basic protein containing a single D-isomer amino acid substitution. / Levy, Robert B; Miller, S.; Garcia, N. M.; Jones, M.; Fisher, G. H.; Man, E. H.

In: Journal of Neuroscience Research, Vol. 25, No. 1, 01.01.1990, p. 29-38.

Research output: Contribution to journalArticle

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