T cell vaccination therapy in an induced model of anti-RNP autoimmune glomerulonephritis

Sapna Trivedi, Yun Juan Zang, Schartess Culpepper, Erica Rosenbaum, Irina Fernandez, Laisel Martinez, Robert W. Hoffman, Eric L. Greidinger

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

To establish the relevance of targeting disease-associated T cells in anti-RNP-associated glomerulonephritis, mice developing nephritis following immunization with U1-70-kd small nuclear ribonucleoprotein (snRNP) were treated with a single dose of irradiated antigen-selected T cell vaccine. T cell receptor usage in nephritic kidneys revealed oligoclonal use of T Cell Receptor V Beta (TRBV) genes as previously found in spleens and lungs of immunized mice with pulmonary disease. The CDR3 regions from T cell isolates showed sequence homology to those in humans with anti-RNP autoimmunity. Following T cell vaccination, urinalysis returned to normal in 5/7 treated mice (71% response rate) whereas all mock-treated mice continued to have an active urinary sediment (Fisher's Exact p = 0.02). An oligoclonal population of T cells homologous to those identified in humans with anti-RNP autoimmunity is implicated in disease pathogenesis, and T cell vaccination is associated with a high rate of clinical improvement in established nephritis.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalClinical Immunology
Volume137
Issue number2
DOIs
StatePublished - Nov 2010

Keywords

  • Lupus
  • Nephritis
  • Ribonucleoproteina
  • T cell
  • Therapy

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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