T cell costimulation by TNFR superfamily (TNFRSF)4 and TNFRSF25 in the context of vaccination

Taylor H. Schreiber, Dietlinde Wolf, Maria Bodero, Louis Gonzalez, Eckhard R. Podack

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

TNFR superfamily (TNFRSF)4 (OX40, CD134) and TNFRSF25 are costimulatory receptors that influence CD4+ and CD8+ T cell responses to cognate Ag. Independently, these receptors have been described to stimulate overlapping functions, including enhanced proliferation and activation for both regulatory T cells (CD4+Foxp3+; Tregs) and conventional T cells (CD4+Foxp3-or CD8+Foxp3-; Tconvs). To determine the relative functionality of TNFRSF4 and TNFRSF25 in T cell immunity, the activity of TNFRSF4 and TNFRS25 agonistic Abs was compared in the context of both traditional protein/adjuvant (OVA/aluminum hydroxide) and CD8+-specific heat shock protein-based (gp96-Ig) vaccine approaches. These studies demonstrate that both TNFRSF4 and TNFRSF25 independently and additively costimulate vaccine-induced CD8+ T cell proliferation following both primary and secondary Ag challenge. In contrast, the activities of TNFRSF4 and TNFRSF25 were observed to be divergent in the costimulation of CD4+ T cell immunity. TNFRSF4 agonists were potent costimulators of OVA/aluminum hydroxide-induced CD4+Tconv proliferation, but they only weakly costimulated Treg proliferation and IgG2a production, whereas TNFRSF25 agonists were strong costimulators of Treg proliferation, producers of IgG1, IgG2a, and IgG2b, and weak costimulators of CD4+ Tconv proliferation. Interestingly, Ag-specific cellular and humoral responses were uncoupled upon secondary immunization, which was dramatically affected by the presence of TNFRSF4 or TNFRSF25 costimulation. These studies highlight the overlapping but nonredundant activities of TNFRSF4 and TNFRSF25 in T cell immunity, which may guide the application of receptor agonistic agents as vaccine adjuvants for infectious disease and tumor immunity.

Original languageEnglish
Pages (from-to)3311-3318
Number of pages8
JournalJournal of Immunology
Volume189
Issue number7
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

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Vaccination
T-Lymphocytes
Immunity
Aluminum Hydroxide
Vaccines
Receptors, Tumor Necrosis Factor, Member 25
Secondary Immunization
Regulatory T-Lymphocytes
Heat-Shock Proteins
Communicable Diseases
Immunoglobulin G
Cell Proliferation
Neoplasms
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

T cell costimulation by TNFR superfamily (TNFRSF)4 and TNFRSF25 in the context of vaccination. / Schreiber, Taylor H.; Wolf, Dietlinde; Bodero, Maria; Gonzalez, Louis; Podack, Eckhard R.

In: Journal of Immunology, Vol. 189, No. 7, 01.10.2012, p. 3311-3318.

Research output: Contribution to journalArticle

Schreiber, TH, Wolf, D, Bodero, M, Gonzalez, L & Podack, ER 2012, 'T cell costimulation by TNFR superfamily (TNFRSF)4 and TNFRSF25 in the context of vaccination', Journal of Immunology, vol. 189, no. 7, pp. 3311-3318. https://doi.org/10.4049/jimmunol.1200597
Schreiber, Taylor H. ; Wolf, Dietlinde ; Bodero, Maria ; Gonzalez, Louis ; Podack, Eckhard R. / T cell costimulation by TNFR superfamily (TNFRSF)4 and TNFRSF25 in the context of vaccination. In: Journal of Immunology. 2012 ; Vol. 189, No. 7. pp. 3311-3318.
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