T cell activation following infection of primary follicle center lymphoma B cells with adenovirus encoding CD154

M. J. Cantwell, W. G. Wierda, I. S. Lossos, R. Levy, T. J. Kipps

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Purified, high-titer adenovirus encoding murine CD154 (Ad-CD154) or human CD154 (Ad-hCD154) was used to infect lymph node cells isolated from patients with follicle center lymphoma. Infection of lymphoma B cells with Ad-CD154 at a multiplicity of infection (MOI) ratio of 100 or higher resulted in high-level transgene expression. Additionally, upon infection of lymphoma B cells, only Ad-CD154 resulted in surface expression of CD154, despite similar, high-level expression of either human or mouse CD154 by HeLa cells infected with Ad-hCD154 or Ad-CD154, respectively. Moreover, infection of lymphoma B cells with Ad-CD154, but not Ad-hCD154 or adenovirus encoding Eschericheria coli beta-galactosidase (Ad-LacZ), induced the neoplastic B cells to express higher levels of immune costimulatory molecules that are required for proficient presentation of antigen to T cells. Consistent with this, we found that Ad-CD154 infected lymphoma B cells could stimulate T cells to proliferate or produce interferon-gamma in allogeneic or autologous mixed lymphocyte interactions. We conclude that lymphoma B cells can be infected with Ad-CD154 and that this significantly enhances their recognition by allogeneic or autologous T cells. As such, Ad-CD154-transduced lymphoma B cells may have potential for the active immune therapy of patients with follicle center lymphoma.

Original languageEnglish (US)
Pages (from-to)1451-1457
Number of pages7
JournalLeukemia
Volume15
Issue number9
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • Adenovirus
  • CD154
  • Follicle center lymphoma

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

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