Abstract
We have identified a single rat monoclonal antibody, G7, that is a potent inducer of interleukin (IL-2) production from all functioning T cell hybridomas as well as from normal T cells. G7 is also mitogenic for normal T cells and is a very effective inducer of IL-2 receptor expression. On fluorescence-activated cell sorter analysis, G7 recognized a pan T-cell antigen. Immunoprecipitation studies demonstraed that G7 recognized a cell surface molecule of 28-32 kD that appeared to be identical to Thy-1 in coprecipitation studies. In addition, G7 precipitated a protein of 50 kD. The possible relationship of the putative molecular complex identified by G7 on murine cells to the molecular complex identified on human T cells with anti-T3 reagents is discussed. In addition, G7 should prove to be a very useful reagent for studying the early events of lymphocyte activation as well as an inducer of lymphokine-rich supernatants.
Original language | English (US) |
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Pages (from-to) | 716-730 |
Number of pages | 15 |
Journal | Journal of Experimental Medicine |
Volume | 159 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 1984 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology