T cell-activating properties of an anti-thy-1 monoclonal antibody possible analogy to OKT3/LEU-4

Kurt C. Gunter, Thomas R. Malek, Ethan M. Shevach

Research output: Contribution to journalArticle

253 Scopus citations

Abstract

We have identified a single rat monoclonal antibody, G7, that is a potent inducer of interleukin (IL-2) production from all functioning T cell hybridomas as well as from normal T cells. G7 is also mitogenic for normal T cells and is a very effective inducer of IL-2 receptor expression. On fluorescence-activated cell sorter analysis, G7 recognized a pan T-cell antigen. Immunoprecipitation studies demonstraed that G7 recognized a cell surface molecule of 28-32 kD that appeared to be identical to Thy-1 in coprecipitation studies. In addition, G7 precipitated a protein of 50 kD. The possible relationship of the putative molecular complex identified by G7 on murine cells to the molecular complex identified on human T cells with anti-T3 reagents is discussed. In addition, G7 should prove to be a very useful reagent for studying the early events of lymphocyte activation as well as an inducer of lymphokine-rich supernatants.

Original languageEnglish (US)
Pages (from-to)716-730
Number of pages15
JournalJournal of Experimental Medicine
Volume159
Issue number3
DOIs
StatePublished - Mar 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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