Systems for therapeutic angiogenesis in tissue engineering

Shay Soker, Marcos Machado, Anthony Atala

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The goals in tissue engineering include the replacement of damaged, injured, or missing body tissues with biologically compatible substitutes. To overcome initial tissue-mass loss, improved vascularization of the regenerated tissue is essential. Two pathways of tissue neovascularization are known: vasculogenesis, the in situ assembly of capillaries from undifferentiated endothelial cells (EC), and angiogenesis, the sprouting of capillaries from preexisting blood vessels. Recent advances in our understanding of the process of blood-vessel growth have provided significant tools for the neovascularization of bioengineered tissues. Several growth factors serve as stimuli for EC proliferation and migration as well as the formation of new blood vessels. They convey their effects via specific receptors expressed on the surface of EC. Vascular epithelial growth factor (VEGF) is a major regulator of neovascularization. VEGF plays a major role in the early development of blood-cell progenitors. Basic fibroblast growth factor (bFGF) was identified as the first angiogenic factor. It is a potent inducer of EC proliferation and blood-vessel growth in vitro and in vivo. VEGF and bFGF have been injected into undervascularized ischemic tissues, resulting in new blood-vessel formation and tissue perfusion. Gene-therapy approaches using VEGF cDNA injection into ischemic tissues have augmented the formation of collateral vessels. Angiogenic factors such as VEGF and bFGF have also been incorporated into bioengineered tissues and have facilitated blood-vessel growth. Other approaches such as prevascularization of the matrix prior to cell seeding and incorporation of EC into the bioengineered tissues have produced encouraging results. This article reviews the process of blood-vessel growth and tissue vascularization, placing emphasis on strategies that can be employed for efficient vascularization of engineered tissues in vitro and in vivo.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalWorld Journal of Urology
Volume18
Issue number1
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Tissue Engineering
Blood Vessels
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Fibroblast Growth Factor 2
Therapeutics
Angiogenesis Inducing Agents
Growth
Cell Proliferation
Helper-Inducer T-Lymphocytes
Genetic Therapy
Cell Movement
Blood Cells
Complementary DNA
Perfusion

ASJC Scopus subject areas

  • Urology

Cite this

Soker, S., Machado, M., & Atala, A. (2000). Systems for therapeutic angiogenesis in tissue engineering. World Journal of Urology, 18(1), 10-18.

Systems for therapeutic angiogenesis in tissue engineering. / Soker, Shay; Machado, Marcos; Atala, Anthony.

In: World Journal of Urology, Vol. 18, No. 1, 01.12.2000, p. 10-18.

Research output: Contribution to journalArticle

Soker, S, Machado, M & Atala, A 2000, 'Systems for therapeutic angiogenesis in tissue engineering', World Journal of Urology, vol. 18, no. 1, pp. 10-18.
Soker S, Machado M, Atala A. Systems for therapeutic angiogenesis in tissue engineering. World Journal of Urology. 2000 Dec 1;18(1):10-18.
Soker, Shay ; Machado, Marcos ; Atala, Anthony. / Systems for therapeutic angiogenesis in tissue engineering. In: World Journal of Urology. 2000 ; Vol. 18, No. 1. pp. 10-18.
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