Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus

J. J David Ho, Miling Wang, Timothy E. Audas, Deukwoo Kwon, Steven K. Carlsson, Sara Timpano, Sonia L. Evagelou, Shaun P Brothers, Mark L Gonzalgo, Jonathan R. Krieger, Xi Chen, James Uniacke, Stephen Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Protein concentrations evolve under greater evolutionary constraint than mRNA levels. Translation efficiency of mRNA represents the chief determinant of basal protein concentrations. This raises a fundamental question of how mRNA and protein levels are coordinated in dynamic systems responding to physiological stimuli. This report examines the contributions of mRNA abundance and translation efficiency to protein output in cells responding to oxygen stimulus. We show that changes in translation efficiencies, and not mRNA levels, represent the major mechanism governing cellular responses to [O2] perturbations. Two distinct cap-dependent protein synthesis machineries select mRNAs for translation: the normoxic eIF4F and the hypoxic eIF4FH. O2-dependent remodeling of translation efficiencies enables cells to produce adaptive translatomes from preexisting mRNA pools. Differences in mRNA expression observed under different [O2] are likely neutral, given that they occur during evolution. We propose that mRNAs contain translation efficiency determinants for their triage by the translation apparatus on [O2] stimulus. Ho et al. show that cells rely on a switch in mRNA translation efficiency, and not mRNA levels, to alter protein output on O2 stimulus.

Original languageEnglish (US)
Pages (from-to)1293-1300
Number of pages8
JournalCell Reports
Volume14
Issue number6
DOIs
StatePublished - Feb 16 2016

Fingerprint

Protein Biosynthesis
Oxygen
Messenger RNA
Proteins
Triage
Dynamical systems
Switches

Keywords

  • Cancer
  • EIF4E
  • EIF4E2
  • EIF4F
  • HIF
  • Hypoxia
  • Oxygen
  • RNA sequencing
  • SILAC
  • Translation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ho, J. J. D., Wang, M., Audas, T. E., Kwon, D., Carlsson, S. K., Timpano, S., ... Lee, S. (2016). Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus. Cell Reports, 14(6), 1293-1300. https://doi.org/10.1016/j.celrep.2016.01.036

Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus. / Ho, J. J David; Wang, Miling; Audas, Timothy E.; Kwon, Deukwoo; Carlsson, Steven K.; Timpano, Sara; Evagelou, Sonia L.; Brothers, Shaun P; Gonzalgo, Mark L; Krieger, Jonathan R.; Chen, Xi; Uniacke, James; Lee, Stephen.

In: Cell Reports, Vol. 14, No. 6, 16.02.2016, p. 1293-1300.

Research output: Contribution to journalArticle

Ho, JJD, Wang, M, Audas, TE, Kwon, D, Carlsson, SK, Timpano, S, Evagelou, SL, Brothers, SP, Gonzalgo, ML, Krieger, JR, Chen, X, Uniacke, J & Lee, S 2016, 'Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus', Cell Reports, vol. 14, no. 6, pp. 1293-1300. https://doi.org/10.1016/j.celrep.2016.01.036
Ho JJD, Wang M, Audas TE, Kwon D, Carlsson SK, Timpano S et al. Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus. Cell Reports. 2016 Feb 16;14(6):1293-1300. https://doi.org/10.1016/j.celrep.2016.01.036
Ho, J. J David ; Wang, Miling ; Audas, Timothy E. ; Kwon, Deukwoo ; Carlsson, Steven K. ; Timpano, Sara ; Evagelou, Sonia L. ; Brothers, Shaun P ; Gonzalgo, Mark L ; Krieger, Jonathan R. ; Chen, Xi ; Uniacke, James ; Lee, Stephen. / Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus. In: Cell Reports. 2016 ; Vol. 14, No. 6. pp. 1293-1300.
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