Systemic reactivation of otitis media with effusion in a rat model

B. S. Jewett, J. P. Prazma, S. E. Hunter, A. S. Rose, J. M. Clark, B. R. Sartor, H. C. Pillsbury

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

OBJECTIVE: This study addresses the interaction of bacterial antigens, specifically peptidoglycan-polysaccharide (PG-PS) and lipopolysaccharide (LPS), in the induction and reactivation of mucoid middle ear effusions. METHODS: Twenty-seven rats underwent eustachian tube obstruction before inoculation of the middle ear bulla with PG-PS. Three weeks later, after resolution of all middle ear effusions, 6 rats were randomly selected and euthanized as the first control group (control I). The remaining 21 animals were randomly assigned to 3 groups that received intravenous Injections of Krebs Ringer (control II), PG-PS, and LPS, respectively. These rats were euthanized 2 days after Intravenous Injection. Middle ear mucin production and histologic changes were measured in all animals. RESULTS: The mean concentrations of mucin were 0.94 ± 0.52 mg/mL, 0.41 ± 0.87 mg/mL, 16.33 ± 3.67 mg/mL, and 1.15 ± 0.41 mg/mL in the control I, control II, PG-PS, and LPS groups, respectively. Thus the mean concentration of mucin in the middle ear lavage samples was significantly greater in rats that were injected intravenously with PG-PS than in rats in other groups (P < 0.05). Histologic analyses demonstrated a greater degree of goblet cell hyperplasia in the PG- PS group than in other groups. CONCLUSIONS: This is the first animal model of recurring otitis media with effusion in which a systemic injection of PG-PS was used to reactivate a middle ear effusion. In rats previously primed with a transtympanic injection of PG-PS. This study suggests that after otitis media with effusion has resolved, it may be reactivated by the presence of bacterial antigens and/or cytokines in the systemic circulation.

Original languageEnglish (US)
Pages (from-to)7-12
Number of pages6
JournalOtolaryngology - Head and Neck Surgery
Volume121
Issue number1
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Systemic reactivation of otitis media with effusion in a rat model'. Together they form a unique fingerprint.

  • Cite this

    Jewett, B. S., Prazma, J. P., Hunter, S. E., Rose, A. S., Clark, J. M., Sartor, B. R., & Pillsbury, H. C. (1999). Systemic reactivation of otitis media with effusion in a rat model. Otolaryngology - Head and Neck Surgery, 121(1), 7-12. https://doi.org/10.1016/S0194-5998(99)70114-2