Systemic overexpression of interleukin-10 fails to protect allogeneic islet transplants in nonobese diabetic mice

Y. Clare Zhang, Antonello Pileggi, R. Damans Molano, Clive Wasserfall, Martha Campbell-Thompson, Camilla Ricordi, Mark A. Atkinson, Luca Inverardi

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Interleukin (IL)-10 has proven effective in various allogeneic transplantation models and for preventing recurrent autoimmune rejection of syngeneic islets in NOD mice. Therefore, we evaluated systemic IL-10 overexpression on allogeneic islet graft survival. Diabetic NOD mice received a single injection of recombinant adeno-associated virus (rAAV) serotype 2 encoding murine IL-10 (rAAV-IL-10) four weeks prior to renal subcasular islet transplantation. In a model having both autoimmune and allogeneic responses, IL-10 failed to protect C57BL/6 islets in spontaneously diabetic NOD mice. In an allograft model (C57BL/6 islets into young male streptozotocin-induced diabetic NOD mice), long-term (i.e., >169 days) islet survival was only seen in 2 of 14 rAAV-IL-10 treated mice. These failures occurred despite in vivo IL-10 production at transplant previously associated with protection of syngeneic islet grafts in NOD mice. Thus, IL-10 appears insufficient in protecting transplanted islet cells from allogeneic rejection and suggests important mechanistic variances between alloreactivity and autoimmunity in terms islet graft loss.

Original languageEnglish (US)
Pages (from-to)530-533
Number of pages4
JournalTransplantation
Volume80
Issue number4
DOIs
StatePublished - Aug 27 2005

Keywords

  • Adeno-associated virus
  • Allogeneic
  • Interleukin-10
  • Islet transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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    Zhang, Y. C., Pileggi, A., Molano, R. D., Wasserfall, C., Campbell-Thompson, M., Ricordi, C., Atkinson, M. A., & Inverardi, L. (2005). Systemic overexpression of interleukin-10 fails to protect allogeneic islet transplants in nonobese diabetic mice. Transplantation, 80(4), 530-533. https://doi.org/10.1097/01.tp.0000168212.53172.06