Systemic ovalbumin sensitization downregulates norepinephrine uptake by rabbit aortic smooth muscle cells

Gabor Horvath, Aliza Torbati, Gregory E Conner, Matthias A Salathe, Adam Wanner

Research output: Contribution to journalArticle

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Abstract

Norepinephrine (NE) concentration at α-adrenergic receptors is partially regulated by steroid-sensitive, extraneuronal catecholamine uptake (uptake-2). Because α1-adrenergic agonist-and glucocorticosteroid (GS)-induced bronchial vasoconstriction is enhanced in individuals with asthma, atopy could be associated with decreased uptake-2 by vascular smooth muscle cells (SMCs). We therefore evaluated whether NE uptake and its specific transporter messenger RNA (mRNA) were reduced in aortic SMCs of rabbits systemically sensitized with ovalbumin (OVA). NE uptake was measured using a semiquantitative fluorescence microscopic method. Corticosterone and O-methyl-isoprenaline, but not desipramine, co-incubation (1 μM each) for 20 min decreased NE uptake into SMCs, an inhibitor profile indicative of extraneuronal monoamine transporter (EMT). In OVA-sensitized rabbits, NE uptake was 25.9 ± 4.5% (mean ± SEM) lower than in control animals (P < 0.05). Sensitized serum had no effect on NE uptake into naive SMCs. EMT mRNA expression was measured in aortic smooth muscle, using multiplex reverse transcriptase-polymerase chain reaction. In OVA-sensitized rabbits, expression was 61.1 ± 16.4% lower than in control animals (P < 0.05). These data demonstrate that NE uptake by aortic SMCs is impaired in atopic rabbits, and associated with a decreased transporter mRNA expression. The same mechanism may operate in bronchial arteries in individuals with asthma.

Original languageEnglish
Pages (from-to)746-751
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume27
Issue number6
StatePublished - Dec 1 2002

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Ovalbumin
Smooth Muscle Myocytes
Muscle
Norepinephrine
Down-Regulation
Cells
Rabbits
Messenger RNA
Animals
Asthma
Bronchial Arteries
Adrenergic Agonists
Desipramine
Multiplex Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Corticosterone
Vasoconstriction
Reverse Transcriptase Polymerase Chain Reaction
Vascular Smooth Muscle

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Systemic ovalbumin sensitization downregulates norepinephrine uptake by rabbit aortic smooth muscle cells. / Horvath, Gabor; Torbati, Aliza; Conner, Gregory E; Salathe, Matthias A; Wanner, Adam.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 27, No. 6, 01.12.2002, p. 746-751.

Research output: Contribution to journalArticle

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