TY - JOUR
T1 - Systemic hypertension induces disparate localized left ventricular action potential lengthening and altered sensitivity to verapamil in left ventricular myocardium
AU - Cameron, John S.
AU - Miller, Linda S.
AU - Kimura, Shinichi
AU - Kaiser, Charles J.
AU - Campbell, David R.
AU - Kozlovskis, Patricia L.
AU - Gaide, Marion S.
AU - Myerburg, Robert J.
AU - Bassett, Arthur L.
N1 - Funding Information:
Lengthening of action potential duration (APD) in ventricular myocardium is a characteristic response of hemodynamic pressure overload-induced hypertrophy in several mammalian species \[3, 6, 10, 21\]. Microelectrode studies on left ventricle removed from rats subjected to maintained renal hypertension have given rise to the proposal that APD lengthening is due to slowed inactivation of a Ca 2 +-inactivated inward current \[3\]. On the other hand, APD lengthening in chronically pressure overloaded cat right ventricle has been ascribed to slowed activation of an outward K + current \[21\] or changes in * Supported by Research Grants HL-19044 and HL-21735, and by a Grant-in-Aid from the American Heart Association, Florida Affiliate (7/83 AG 216)and an NIH Biomedical Research Support Grant (S07 RR-05363) to Dr John S. Cameron. Drs Cameron, Kozlovskis and Gaide are recipients of NIH New Investigator Research Awards HL-32496, HL-30633, HL-27680, respectively.
PY - 1986/2
Y1 - 1986/2
N2 - Previously, we demonstrated that acute exposure to superfusate containing verapamil suppresses action potential plateau to a greater degree in cells at the tip of the anterior papillary muscle compared to those at its base in normal cat left ventricle (LV) studied in tissue bath. To determine the effects of chronic pressure overload brought about by renal hypertension on (1) papillary muscle electrophysiology and (2) regional sensitivity to verapamil, LV were isolated from cats subjected to unilateral nephrectomy and contralateral kidney wrapping. After 3 months, systemic hypertension (mean increase=60.2±1.4 mmHg) was associated with moderate LV hypertrophy of ≈18% (LV weight/body weight). Transmembrane action potentials recorded from the endocardial surface (including anterior papillary muscle) in pressure overloaded LV in vitro (800 ms stimulus cycle length) showed increased action potential duration at 25, 75 and 90% of repolarization (APD25, APD75 and APD90, respectively) relative to controls (LV from normal and sham operated cats). With respect to the anterior papillary muscle in pressure overloaded LV, APD25, APD75 and APD90 were increased to a greater extent in cells at the base of the muscle compared to those increases observed at the tip (P<0.05). In contrast to its effects in controls, verapamil (2 μg/ml) significantly reduced APD25 at both the base and the tip of the papillary muscle in pressure overloaded preparations, but particularly at the base; also, the disparities between the APD75 or APD90 at tip and base were decreased. Thus, (1) regional electrophysiologic disparities were induced in systemic hypertension, and (2) these differences were subsequently reduced by verapamil. Highly localized disparate effects of verapamil in normal and pressure overloaded LV myocardium indicate the need to assess drug action in diseased preparations.
AB - Previously, we demonstrated that acute exposure to superfusate containing verapamil suppresses action potential plateau to a greater degree in cells at the tip of the anterior papillary muscle compared to those at its base in normal cat left ventricle (LV) studied in tissue bath. To determine the effects of chronic pressure overload brought about by renal hypertension on (1) papillary muscle electrophysiology and (2) regional sensitivity to verapamil, LV were isolated from cats subjected to unilateral nephrectomy and contralateral kidney wrapping. After 3 months, systemic hypertension (mean increase=60.2±1.4 mmHg) was associated with moderate LV hypertrophy of ≈18% (LV weight/body weight). Transmembrane action potentials recorded from the endocardial surface (including anterior papillary muscle) in pressure overloaded LV in vitro (800 ms stimulus cycle length) showed increased action potential duration at 25, 75 and 90% of repolarization (APD25, APD75 and APD90, respectively) relative to controls (LV from normal and sham operated cats). With respect to the anterior papillary muscle in pressure overloaded LV, APD25, APD75 and APD90 were increased to a greater extent in cells at the base of the muscle compared to those increases observed at the tip (P<0.05). In contrast to its effects in controls, verapamil (2 μg/ml) significantly reduced APD25 at both the base and the tip of the papillary muscle in pressure overloaded preparations, but particularly at the base; also, the disparities between the APD75 or APD90 at tip and base were decreased. Thus, (1) regional electrophysiologic disparities were induced in systemic hypertension, and (2) these differences were subsequently reduced by verapamil. Highly localized disparate effects of verapamil in normal and pressure overloaded LV myocardium indicate the need to assess drug action in diseased preparations.
KW - Action potential duration
KW - Anterior papillary muscle
KW - Cardiac electrophysiology
KW - Cat
KW - Left ventricular pressure overload
KW - Microelectrodes
KW - Renal hypertension
KW - Verapamil
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U2 - 10.1016/S0022-2828(86)80469-2
DO - 10.1016/S0022-2828(86)80469-2
M3 - Article
C2 - 3959090
AN - SCOPUS:0022618689
VL - 18
SP - 169
EP - 175
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 2
ER -