Systemic Gene Silencing in Primary T Lymphocytes Using Targeted Lipid Nanoparticles

Srinivas Ramishetti, Ranit Kedmi, Meir Goldsmith, Fransisca Leonard, Andrew G. Sprague, Biana Godin, Michael Gozin, Pieter R. Cullis, Derek M Dykxhoorn, Dan Peer

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Modulating T cell function by down-regulating specific genes using RNA interference (RNAi) holds tremendous potential in advancing targeted therapies in many immune-related disorders including cancer, inflammation, autoimmunity, and viral infections. Hematopoietic cells, in general, and primary T lymphocytes, in particular, are notoriously hard to transfect with small interfering RNAs (siRNAs). Herein, we describe a novel strategy to specifically deliver siRNAs to murine CD4<sup>+</sup> T cells using targeted lipid nanoparticles (tLNPs). To increase the efficacy of siRNA delivery, these tLNPs have been formulated with several lipids designed to improve the stability and efficacy of siRNA delivery. The tLNPs were surface-functionalized with anti-CD4 monoclonal antibody to permit delivery of the siRNAs specifically to CD4<sup>+</sup> T lymphocytes. Ex vivo, tLNPs demonstrated specificity by targeting only primary CD4<sup>+</sup> T lymphocytes and no other cell types. Systemic intravenous administration of these particles led to efficient binding and uptake into CD4<sup>+</sup> T lymphocytes in several anatomical sites including the spleen, inguinal lymph nodes, blood, and the bone marrow. Silencing by tLNPs occurs in a subset of circulating and resting CD4<sup>+</sup> T lymphocytes. Interestingly, we show that tLNP internalization and not endosome escape is a fundamental event that takes place as early as 1 h after systemic administration and determines tLNPs' efficacy. Taken together, these results suggest that tLNPs may open new avenues for the manipulation of T cell functionality and may help to establish RNAi as a therapeutic modality in leukocyte-associated diseases.

Original languageEnglish (US)
Pages (from-to)6706-6716
Number of pages11
JournalACS Nano
Volume9
Issue number7
DOIs
StatePublished - Jul 28 2015

Keywords

  • CD45
  • lipid-based nanoparticles
  • RNA interference
  • T lymphocytes

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Systemic Gene Silencing in Primary T Lymphocytes Using Targeted Lipid Nanoparticles'. Together they form a unique fingerprint.

  • Cite this

    Ramishetti, S., Kedmi, R., Goldsmith, M., Leonard, F., Sprague, A. G., Godin, B., Gozin, M., Cullis, P. R., Dykxhoorn, D. M., & Peer, D. (2015). Systemic Gene Silencing in Primary T Lymphocytes Using Targeted Lipid Nanoparticles. ACS Nano, 9(7), 6706-6716. https://doi.org/10.1021/acsnano.5b02796