Systematic determination of telomerase activity and telomerase length during the progression of human breast cancer in cell culture models

S. Ashraf Imam, Mee S. Kim, Lars Anker, Ram Datar, Ronald E. Law, Clive R. Taylor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The purpose of the study was to determine systematically the expression of telomerase activity and the length of telomere repeat arrays by utilizing two different cell culture models that derive from normal individual donors, and probably represent various stages of human breast oncogenesis in cell culture. The models consist of mortal, non-tumorigenic immortal and tumorigenic immortal human mammary epithelial cell (MEC) lines. Using a recently developed polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP) assay, telomerase activity was undetectable in mortal MEC cells. In contrast, the immortal MEC that were non-tumorigenic or tumorigenic in immunosuppressed athymic mice, showed telomerase activity. The absence of telomerase activity in mortal and its presence in both non-tumorigenic and tumorigenic immortal cell lines did not reflect their proliferative rate, as demonstrated by the similar pattern and intensity of reactivity of these cell lines with anti-Ki 67 antibody which recognizes a human nuclear cell proliferation-associated antigen. Southern blot analyses of Hinf I-digested genomic DNA hybridized with a (TTAGGG)4 probe revealed arrays of telomeric repeat lengths ranging from 3 to 5, 3.5 to 9, 3.2 to 9 or 3 to 15 kilobase pair (kbp) for mortal, nontumorigenic immortal, and tumorigenic immortal or established MEC lines respectively. These results suggest that telomerase activity and stable telomeric repeat lengths may be a molecular phenotype of the early stages in the progression of breast cancer.

Original languageEnglish
Pages (from-to)4435-4441
Number of pages7
JournalAnticancer Research
Volume17
Issue number6 D
StatePublished - Nov 1 1997
Externally publishedYes

Fingerprint

Telomerase
Breast
Cell Culture Techniques
Breast Neoplasms
Epithelial Cells
Cell Line
Telomere
Southern Blotting
Nude Mice
Carcinogenesis
Cell Proliferation
Phenotype
Antigens
Polymerase Chain Reaction
Antibodies
DNA

Keywords

  • Breast cancer
  • Breast oncogenesis
  • Telomerase
  • Telomere

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Imam, S. A., Kim, M. S., Anker, L., Datar, R., Law, R. E., & Taylor, C. R. (1997). Systematic determination of telomerase activity and telomerase length during the progression of human breast cancer in cell culture models. Anticancer Research, 17(6 D), 4435-4441.

Systematic determination of telomerase activity and telomerase length during the progression of human breast cancer in cell culture models. / Imam, S. Ashraf; Kim, Mee S.; Anker, Lars; Datar, Ram; Law, Ronald E.; Taylor, Clive R.

In: Anticancer Research, Vol. 17, No. 6 D, 01.11.1997, p. 4435-4441.

Research output: Contribution to journalArticle

Imam, S. Ashraf ; Kim, Mee S. ; Anker, Lars ; Datar, Ram ; Law, Ronald E. ; Taylor, Clive R. / Systematic determination of telomerase activity and telomerase length during the progression of human breast cancer in cell culture models. In: Anticancer Research. 1997 ; Vol. 17, No. 6 D. pp. 4435-4441.
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