Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications

Eileen Pedraza, Ann Christina Brady, Christopher A. Fraker, Cherie L. Stabler

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Macroporous, biostable scaffolds with controlled porous architecture were prepared from poly(dimethylsiloxane) (PDMS) using sodium chloride particles and a solvent casting and particulate leaching technique. The effect of particulate size range and overall porosity on the resulting structure was evaluated. Results found 90% v/v scaffolds and particulate ranges above 100 μm to have the most optimal open framework and porosity. Resulting hydrophobic PDMS scaffolds were coated with fibronectin and evaluated as a platform for adherent cell culture using human mesenchymal stem cells. Biocompatibility of PDMS scaffolds was also evaluated in a rodent model, where implants were found to be highly biocompatible and biostable, with positive extracellular matrix deposition throughout the scaffold. These results demonstrate the suitability of macroporous PDMS scaffolds for tissue engineering applications where strong integration with the host is desired.

Original languageEnglish (US)
Pages (from-to)1041-1056
Number of pages16
JournalJournal of Biomaterials Science, Polymer Edition
Volume24
Issue number9
DOIs
StatePublished - Jun 1 2013

Keywords

  • biocompatibility
  • cellular transplantation
  • macroporous
  • matrix
  • mesenchymal stem cells
  • poly(dimethylsiloxane)

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications'. Together they form a unique fingerprint.

Cite this