Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid

David H. Coy, Esther J. Coy, Tommie W. Redding, Andrew V Schally

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A method of preparing luteinizing hormone-releasing hormone (LH-RH) pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, by a combination of solid-phase and classical reactions was employed to conveniently synthesize a tritium-labelled hormone by incorporation of 4-[3H]-pyroglutamic acid into position I of the peptide chain. The tritiated LH-RH possessed a specific radioactivity of 18.3 Ci/mmole and a maximal biological potency.

Original languageEnglish
Pages (from-to)866-871
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume50
Issue number3
DOIs
StatePublished - Feb 5 1973
Externally publishedYes

Fingerprint

Pyrrolidonecarboxylic Acid
Tritium
Gonadotropin-Releasing Hormone
Radioactivity
Hormones
Peptides
leucyl-arginyl-prolyl-glycinamide
pyroglutamylhistidine
pyroglutamyltryptophan

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis of luteinizing hormone-releasing hormone containing tritium-labelled pyroglutamic acid. / Coy, David H.; Coy, Esther J.; Redding, Tommie W.; Schally, Andrew V.

In: Biochemical and Biophysical Research Communications, Vol. 50, No. 3, 05.02.1973, p. 866-871.

Research output: Contribution to journalArticle

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