Synthesis, crystal structure and photo-induced DNA cleavage activity of ternary copper(II) complexes of NSO-donor Schiff bases and NN-donor heterocyclic ligands

Shanta Dhar, Munirathinam Nethaji, Akhil R. Chakravarty

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

New ternary copper(II) complexes [CuLnB](ClO4) (1-3), where HLn is the NSO donor Schiff base derived from the condensation of 2-mercaptoethylamine hydrochloride with salicylaldehyde (HL1) or 2-hydroxy-3-methoxybenzaldehyde (HL2) and B is NN-donor heterocyclic base like 2,2′-bipyridine (bpy, 1), 1,10-phenanthroline (phen, 2) or 2,9-dimethyl-1,10-phenanthroline (dmp, 3), are prepared, structurally characterized by X-ray crystallography and their DNA cleavage activity studied. The complexes show distorted square-pyramidal (4 + 1) CuN3OS coordination geometry in which the NSO-donor Schiff base is bonded at the basal plane and the NN-donor heterocyclic base displays axial-equatorial mode of bonding [Cu-S distance: ∼2.4 Å]. The one-electron paramagnetic (μeff = ∼1.9 μB) complexes display axial EPR spectra in DMF glass at 77 K giving g = ∼2.2 (A = 162 G) and g = ∼2.0, indicating {dx2-y2}1 ground state. The complexes exhibit visible spectral d-d band in MeCN near 650 nm and two charge transfer bands near 400 nm. Complexes 1 and 2 display quasireversible cyclic voltammetric response in DMF-Tris buffer (1:4 v/v, pH 7.2) for the Cu(II)/Cu(I) couple at ca. -0.1 V vs. SCE. Complex 3 exhibits an irreversible reduction process forming [CuI(dmp)2] +. Binding of 1-3 to calf thymus DNA shows the relative order: 2 (phen) ≥ 3 (dmp) > 1 (bpy). Complex 2 efficiently cleaves supercoiled pUC19 DNA in the presence of mercaptopropionic acid (MPA) forming hydroxyl radical or on irradiation with light of 312, 532 and 632.8 nm wavelength in a type-II process. Complexes 1 and 3 are cleavage inactive.

Original languageEnglish (US)
Pages (from-to)2437-2444
Number of pages8
JournalInorganica Chimica Acta
Volume358
Issue number7
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

Fingerprint

Schiff Bases
imines
Copper
cleavage
DNA
deoxyribonucleic acid
Crystal structure
Ligands
Cysteamine
copper
ligands
Tromethamine
crystal structure
X ray crystallography
synthesis
Hydroxyl Radical
Ground state
Paramagnetic resonance
Charge transfer
Thymus

Keywords

  • Crystal structure
  • Heterocyclic base
  • NSO-donor Schiff base
  • Photo-induced DNA cleavage
  • Ternary copper(II) complex

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry
  • Materials Chemistry

Cite this

Synthesis, crystal structure and photo-induced DNA cleavage activity of ternary copper(II) complexes of NSO-donor Schiff bases and NN-donor heterocyclic ligands. / Dhar, Shanta; Nethaji, Munirathinam; Chakravarty, Akhil R.

In: Inorganica Chimica Acta, Vol. 358, No. 7, 15.04.2005, p. 2437-2444.

Research output: Contribution to journalArticle

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title = "Synthesis, crystal structure and photo-induced DNA cleavage activity of ternary copper(II) complexes of NSO-donor Schiff bases and NN-donor heterocyclic ligands",
abstract = "New ternary copper(II) complexes [CuLnB](ClO4) (1-3), where HLn is the NSO donor Schiff base derived from the condensation of 2-mercaptoethylamine hydrochloride with salicylaldehyde (HL1) or 2-hydroxy-3-methoxybenzaldehyde (HL2) and B is NN-donor heterocyclic base like 2,2′-bipyridine (bpy, 1), 1,10-phenanthroline (phen, 2) or 2,9-dimethyl-1,10-phenanthroline (dmp, 3), are prepared, structurally characterized by X-ray crystallography and their DNA cleavage activity studied. The complexes show distorted square-pyramidal (4 + 1) CuN3OS coordination geometry in which the NSO-donor Schiff base is bonded at the basal plane and the NN-donor heterocyclic base displays axial-equatorial mode of bonding [Cu-S distance: ∼2.4 {\AA}]. The one-electron paramagnetic (μeff = ∼1.9 μB) complexes display axial EPR spectra in DMF glass at 77 K giving g∥ = ∼2.2 (A ∥ = 162 G) and g⊥ = ∼2.0, indicating {dx2-y2}1 ground state. The complexes exhibit visible spectral d-d band in MeCN near 650 nm and two charge transfer bands near 400 nm. Complexes 1 and 2 display quasireversible cyclic voltammetric response in DMF-Tris buffer (1:4 v/v, pH 7.2) for the Cu(II)/Cu(I) couple at ca. -0.1 V vs. SCE. Complex 3 exhibits an irreversible reduction process forming [CuI(dmp)2] +. Binding of 1-3 to calf thymus DNA shows the relative order: 2 (phen) ≥ 3 (dmp) > 1 (bpy). Complex 2 efficiently cleaves supercoiled pUC19 DNA in the presence of mercaptopropionic acid (MPA) forming hydroxyl radical or on irradiation with light of 312, 532 and 632.8 nm wavelength in a type-II process. Complexes 1 and 3 are cleavage inactive.",
keywords = "Crystal structure, Heterocyclic base, NSO-donor Schiff base, Photo-induced DNA cleavage, Ternary copper(II) complex",
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T1 - Synthesis, crystal structure and photo-induced DNA cleavage activity of ternary copper(II) complexes of NSO-donor Schiff bases and NN-donor heterocyclic ligands

AU - Dhar, Shanta

AU - Nethaji, Munirathinam

AU - Chakravarty, Akhil R.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - New ternary copper(II) complexes [CuLnB](ClO4) (1-3), where HLn is the NSO donor Schiff base derived from the condensation of 2-mercaptoethylamine hydrochloride with salicylaldehyde (HL1) or 2-hydroxy-3-methoxybenzaldehyde (HL2) and B is NN-donor heterocyclic base like 2,2′-bipyridine (bpy, 1), 1,10-phenanthroline (phen, 2) or 2,9-dimethyl-1,10-phenanthroline (dmp, 3), are prepared, structurally characterized by X-ray crystallography and their DNA cleavage activity studied. The complexes show distorted square-pyramidal (4 + 1) CuN3OS coordination geometry in which the NSO-donor Schiff base is bonded at the basal plane and the NN-donor heterocyclic base displays axial-equatorial mode of bonding [Cu-S distance: ∼2.4 Å]. The one-electron paramagnetic (μeff = ∼1.9 μB) complexes display axial EPR spectra in DMF glass at 77 K giving g∥ = ∼2.2 (A ∥ = 162 G) and g⊥ = ∼2.0, indicating {dx2-y2}1 ground state. The complexes exhibit visible spectral d-d band in MeCN near 650 nm and two charge transfer bands near 400 nm. Complexes 1 and 2 display quasireversible cyclic voltammetric response in DMF-Tris buffer (1:4 v/v, pH 7.2) for the Cu(II)/Cu(I) couple at ca. -0.1 V vs. SCE. Complex 3 exhibits an irreversible reduction process forming [CuI(dmp)2] +. Binding of 1-3 to calf thymus DNA shows the relative order: 2 (phen) ≥ 3 (dmp) > 1 (bpy). Complex 2 efficiently cleaves supercoiled pUC19 DNA in the presence of mercaptopropionic acid (MPA) forming hydroxyl radical or on irradiation with light of 312, 532 and 632.8 nm wavelength in a type-II process. Complexes 1 and 3 are cleavage inactive.

AB - New ternary copper(II) complexes [CuLnB](ClO4) (1-3), where HLn is the NSO donor Schiff base derived from the condensation of 2-mercaptoethylamine hydrochloride with salicylaldehyde (HL1) or 2-hydroxy-3-methoxybenzaldehyde (HL2) and B is NN-donor heterocyclic base like 2,2′-bipyridine (bpy, 1), 1,10-phenanthroline (phen, 2) or 2,9-dimethyl-1,10-phenanthroline (dmp, 3), are prepared, structurally characterized by X-ray crystallography and their DNA cleavage activity studied. The complexes show distorted square-pyramidal (4 + 1) CuN3OS coordination geometry in which the NSO-donor Schiff base is bonded at the basal plane and the NN-donor heterocyclic base displays axial-equatorial mode of bonding [Cu-S distance: ∼2.4 Å]. The one-electron paramagnetic (μeff = ∼1.9 μB) complexes display axial EPR spectra in DMF glass at 77 K giving g∥ = ∼2.2 (A ∥ = 162 G) and g⊥ = ∼2.0, indicating {dx2-y2}1 ground state. The complexes exhibit visible spectral d-d band in MeCN near 650 nm and two charge transfer bands near 400 nm. Complexes 1 and 2 display quasireversible cyclic voltammetric response in DMF-Tris buffer (1:4 v/v, pH 7.2) for the Cu(II)/Cu(I) couple at ca. -0.1 V vs. SCE. Complex 3 exhibits an irreversible reduction process forming [CuI(dmp)2] +. Binding of 1-3 to calf thymus DNA shows the relative order: 2 (phen) ≥ 3 (dmp) > 1 (bpy). Complex 2 efficiently cleaves supercoiled pUC19 DNA in the presence of mercaptopropionic acid (MPA) forming hydroxyl radical or on irradiation with light of 312, 532 and 632.8 nm wavelength in a type-II process. Complexes 1 and 3 are cleavage inactive.

KW - Crystal structure

KW - Heterocyclic base

KW - NSO-donor Schiff base

KW - Photo-induced DNA cleavage

KW - Ternary copper(II) complex

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