Synthesis and release of insulinlike growth factor I by mesangial cells in culture

F. G. Conti, L. J. Striker, Sharon Elliot, D. Andreani, G. E. Striker

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Mesangial cell proliferation is a common hallmark of many glomerular diseases. The exact mechanisms inducing cell proliferation in glomerulosclerosis are not completely understood, and it remains to be determined whether growth factors play a role in this process. Insulinlike growth factor I (IGF I) has been shown to be synthesized in the kidney, and glomerular mesangial cells have receptors for and exhibit mitogenic response to IGF I. We found that mouse glomerular mesangial cells in culture synthesized and released into the culture medium a molecule with immunological and biological features of IGF I. This molecular specifically bound to mesangial cell IGF I receptors; high-pressure liquid chromatographic analysis provided further evidence of its similarity to human recombinant IGF I. Mesangial cells released into the culture medium 6 ng/106 cells of IGF I-like material per 24 h in a time-dependent and actinomycin-D inhibitable fashion. These data suggest that IGF I might be locally released by mesangial cells in the glomerulus and act in an autocrine and paracrine fashion.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume255
Issue number6
StatePublished - Dec 1 1988
Externally publishedYes

Fingerprint

Mesangial Cells
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Insulin-Like Growth Factor I
Culture Media
Cell Proliferation
Growth Factor Receptors
Dactinomycin
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Physiology

Cite this

Synthesis and release of insulinlike growth factor I by mesangial cells in culture. / Conti, F. G.; Striker, L. J.; Elliot, Sharon; Andreani, D.; Striker, G. E.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 255, No. 6, 01.12.1988.

Research output: Contribution to journalArticle

@article{a8bda795fb134a09bd42718f158b90ec,
title = "Synthesis and release of insulinlike growth factor I by mesangial cells in culture",
abstract = "Mesangial cell proliferation is a common hallmark of many glomerular diseases. The exact mechanisms inducing cell proliferation in glomerulosclerosis are not completely understood, and it remains to be determined whether growth factors play a role in this process. Insulinlike growth factor I (IGF I) has been shown to be synthesized in the kidney, and glomerular mesangial cells have receptors for and exhibit mitogenic response to IGF I. We found that mouse glomerular mesangial cells in culture synthesized and released into the culture medium a molecule with immunological and biological features of IGF I. This molecular specifically bound to mesangial cell IGF I receptors; high-pressure liquid chromatographic analysis provided further evidence of its similarity to human recombinant IGF I. Mesangial cells released into the culture medium 6 ng/106 cells of IGF I-like material per 24 h in a time-dependent and actinomycin-D inhibitable fashion. These data suggest that IGF I might be locally released by mesangial cells in the glomerulus and act in an autocrine and paracrine fashion.",
author = "Conti, {F. G.} and Striker, {L. J.} and Sharon Elliot and D. Andreani and Striker, {G. E.}",
year = "1988",
month = "12",
day = "1",
language = "English",
volume = "255",
journal = "American Journal of Physiology - Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - Synthesis and release of insulinlike growth factor I by mesangial cells in culture

AU - Conti, F. G.

AU - Striker, L. J.

AU - Elliot, Sharon

AU - Andreani, D.

AU - Striker, G. E.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - Mesangial cell proliferation is a common hallmark of many glomerular diseases. The exact mechanisms inducing cell proliferation in glomerulosclerosis are not completely understood, and it remains to be determined whether growth factors play a role in this process. Insulinlike growth factor I (IGF I) has been shown to be synthesized in the kidney, and glomerular mesangial cells have receptors for and exhibit mitogenic response to IGF I. We found that mouse glomerular mesangial cells in culture synthesized and released into the culture medium a molecule with immunological and biological features of IGF I. This molecular specifically bound to mesangial cell IGF I receptors; high-pressure liquid chromatographic analysis provided further evidence of its similarity to human recombinant IGF I. Mesangial cells released into the culture medium 6 ng/106 cells of IGF I-like material per 24 h in a time-dependent and actinomycin-D inhibitable fashion. These data suggest that IGF I might be locally released by mesangial cells in the glomerulus and act in an autocrine and paracrine fashion.

AB - Mesangial cell proliferation is a common hallmark of many glomerular diseases. The exact mechanisms inducing cell proliferation in glomerulosclerosis are not completely understood, and it remains to be determined whether growth factors play a role in this process. Insulinlike growth factor I (IGF I) has been shown to be synthesized in the kidney, and glomerular mesangial cells have receptors for and exhibit mitogenic response to IGF I. We found that mouse glomerular mesangial cells in culture synthesized and released into the culture medium a molecule with immunological and biological features of IGF I. This molecular specifically bound to mesangial cell IGF I receptors; high-pressure liquid chromatographic analysis provided further evidence of its similarity to human recombinant IGF I. Mesangial cells released into the culture medium 6 ng/106 cells of IGF I-like material per 24 h in a time-dependent and actinomycin-D inhibitable fashion. These data suggest that IGF I might be locally released by mesangial cells in the glomerulus and act in an autocrine and paracrine fashion.

UR - http://www.scopus.com/inward/record.url?scp=0024266356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024266356&partnerID=8YFLogxK

M3 - Article

C2 - 2974247

AN - SCOPUS:0024266356

VL - 255

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 6

ER -