Synthesis and biological properties of [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity

David H. Coy, Esther J. Coy, Andrew V Schally, Jesus Vilchez-Martinez, Yoshihiro Hirotsu, Akira Arimura

Research output: Contribution to journalArticle

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Abstract

A nonapeptide analog of luteinizing hormone-releasing hormone (LH-RH), [D-Ala6, des-Gly-NH2 10]-LH-RH ethylamide, was prepared by solid-phase methodology. The peptide was assayed against LH-RH in two in vivo systems and was found to be many times more potent than the naturally occurring hormone. In one of the tests, based on elevation of LH and FSH levels after infusion into immature male rats, the analog showed LH-releasing activity of 1600% and FSH-releasing activity of 1200% compared to LH-RH.

Original languageEnglish
Pages (from-to)335-340
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume57
Issue number2
DOIs
StatePublished - Mar 25 1974
Externally publishedYes

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Gonadotropin-Releasing Hormone
Peptides
Rats
Hormones
des-Gly(10)-Ala(6)-lHRH ethylamide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis and biological properties of [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity. / Coy, David H.; Coy, Esther J.; Schally, Andrew V; Vilchez-Martinez, Jesus; Hirotsu, Yoshihiro; Arimura, Akira.

In: Biochemical and Biophysical Research Communications, Vol. 57, No. 2, 25.03.1974, p. 335-340.

Research output: Contribution to journalArticle

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abstract = "A nonapeptide analog of luteinizing hormone-releasing hormone (LH-RH), [D-Ala6, des-Gly-NH2 10]-LH-RH ethylamide, was prepared by solid-phase methodology. The peptide was assayed against LH-RH in two in vivo systems and was found to be many times more potent than the naturally occurring hormone. In one of the tests, based on elevation of LH and FSH levels after infusion into immature male rats, the analog showed LH-releasing activity of 1600{\%} and FSH-releasing activity of 1200{\%} compared to LH-RH.",
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