Synthesis and biological properties of [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity

David H. Coy; Esther J. Coy; Andrew V. Schally; Jesus Vilchez-Martinez; Yoshihiro Hirotsu; Akira Arimura

doi:10.1016/0006-291X(74)90934-6

Synthesis and biological properties of [D-Ala-6, des-Gly-NH₂-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity

David H. Coy, Esther J. Coy, Andrew V. Schally, Jesus Vilchez-Martinez, Yoshihiro Hirotsu, Akira Arimura

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

A nonapeptide analog of luteinizing hormone-releasing hormone (LH-RH), [D-Ala⁶, des-Gly-NH₂ ¹⁰]-LH-RH ethylamide, was prepared by solid-phase methodology. The peptide was assayed against LH-RH in two in vivo systems and was found to be many times more potent than the naturally occurring hormone. In one of the tests, based on elevation of LH and FSH levels after infusion into immature male rats, the analog showed LH-releasing activity of 1600% and FSH-releasing activity of 1200% compared to LH-RH.

Original language	English (US)
Pages (from-to)	335-340
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(74)90934-6
State	Published - Mar 25 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Synthesis and biological properties of [D-Ala-6, des-Gly-NH₂-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity. / Coy, David H.; Coy, Esther J.; Schally, Andrew V.; Vilchez-Martinez, Jesus; Hirotsu, Yoshihiro; Arimura, Akira.

In: Biochemical and biophysical research communications, Vol. 57, No. 2, 25.03.1974, p. 335-340.

Research output: Contribution to journal › Article › peer-review

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title = "Synthesis and biological properties of [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide, a peptide with greatly enhanced LH- and FSH-releasing activity",

abstract = "A nonapeptide analog of luteinizing hormone-releasing hormone (LH-RH), [D-Ala6, des-Gly-NH2 10]-LH-RH ethylamide, was prepared by solid-phase methodology. The peptide was assayed against LH-RH in two in vivo systems and was found to be many times more potent than the naturally occurring hormone. In one of the tests, based on elevation of LH and FSH levels after infusion into immature male rats, the analog showed LH-releasing activity of 1600% and FSH-releasing activity of 1200% compared to LH-RH.",

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AU - Hirotsu, Yoshihiro

AU - Arimura, Akira

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