Syntheses by the conventional method are described of [Gln8]-LH-RH, [Leu8]-LH-RH and [Pro8] [Arg9]-LH-RH. These peptides were purified by column chromatography on CM-Sephadex and gelfiltration on Sephadex G-25 and proved to be homogeneous. The LH-RH and FSH-RH activities of these peptides were compared with those of natural LH-RH in vivo and in vitro. [Gln8]-LH-RH had significant LH- and FSH-RH activities, while [Leu8]-LH-RH and [Pro8] [Arg9]-LH-RH had lower activities.
|Original language||English (US)|
|Number of pages||12|
|Journal||Biochemical and biophysical research communications|
|State||Published - Dec 4 1972|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology