Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies

Jonah B. Sacha, David Watkins

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The development of an HIV vaccine will require a more precise understanding of the immunological and virological underpinnings of HIV infection. Magnetofection, the process of magnetizing HIV by coupling it to ferrous nanoparticles and coordinating infection using a magnetic field, synchronizes the viral replication cycle at attachment while recapitulating the events of natural infection. Although spinoculation also concentrates virus onto target cells to increase infection, it does not synchronize infection. The synchronization of HIV infection in vitro facilitates the study of events in the viral replication cycle and the antiviral immune response on timelines previously impossible. Furthermore, by infecting a high percentage of cells in a short time frame, magnetofection increases the throughput of in vitro assays. Once a virus stock is generated, magnetofection of target cells is rapid, requiring only 1-2 h. Here we present a detailed protocol for this assay and review its applications for studying the immune response to HIV.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalNature Protocols
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Immunology
Virology
Allergy and Immunology
Viruses
HIV Infections
Assays
Vaccines
AIDS Vaccines
Infection
Antiviral Agents
Synchronization
Throughput
HIV
Magnetic fields
Nanoparticles
Magnetic Fields
In Vitro Techniques
TimeLine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies. / Sacha, Jonah B.; Watkins, David.

In: Nature Protocols, Vol. 5, No. 2, 01.02.2010, p. 239-246.

Research output: Contribution to journalArticle

@article{64a78dfe57804a8185967bf253af24cd,
title = "Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies",
abstract = "The development of an HIV vaccine will require a more precise understanding of the immunological and virological underpinnings of HIV infection. Magnetofection, the process of magnetizing HIV by coupling it to ferrous nanoparticles and coordinating infection using a magnetic field, synchronizes the viral replication cycle at attachment while recapitulating the events of natural infection. Although spinoculation also concentrates virus onto target cells to increase infection, it does not synchronize infection. The synchronization of HIV infection in vitro facilitates the study of events in the viral replication cycle and the antiviral immune response on timelines previously impossible. Furthermore, by infecting a high percentage of cells in a short time frame, magnetofection increases the throughput of in vitro assays. Once a virus stock is generated, magnetofection of target cells is rapid, requiring only 1-2 h. Here we present a detailed protocol for this assay and review its applications for studying the immune response to HIV.",
author = "Sacha, {Jonah B.} and David Watkins",
year = "2010",
month = "2",
day = "1",
doi = "10.1038/nprot.2009.227",
language = "English",
volume = "5",
pages = "239--246",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies

AU - Sacha, Jonah B.

AU - Watkins, David

PY - 2010/2/1

Y1 - 2010/2/1

N2 - The development of an HIV vaccine will require a more precise understanding of the immunological and virological underpinnings of HIV infection. Magnetofection, the process of magnetizing HIV by coupling it to ferrous nanoparticles and coordinating infection using a magnetic field, synchronizes the viral replication cycle at attachment while recapitulating the events of natural infection. Although spinoculation also concentrates virus onto target cells to increase infection, it does not synchronize infection. The synchronization of HIV infection in vitro facilitates the study of events in the viral replication cycle and the antiviral immune response on timelines previously impossible. Furthermore, by infecting a high percentage of cells in a short time frame, magnetofection increases the throughput of in vitro assays. Once a virus stock is generated, magnetofection of target cells is rapid, requiring only 1-2 h. Here we present a detailed protocol for this assay and review its applications for studying the immune response to HIV.

AB - The development of an HIV vaccine will require a more precise understanding of the immunological and virological underpinnings of HIV infection. Magnetofection, the process of magnetizing HIV by coupling it to ferrous nanoparticles and coordinating infection using a magnetic field, synchronizes the viral replication cycle at attachment while recapitulating the events of natural infection. Although spinoculation also concentrates virus onto target cells to increase infection, it does not synchronize infection. The synchronization of HIV infection in vitro facilitates the study of events in the viral replication cycle and the antiviral immune response on timelines previously impossible. Furthermore, by infecting a high percentage of cells in a short time frame, magnetofection increases the throughput of in vitro assays. Once a virus stock is generated, magnetofection of target cells is rapid, requiring only 1-2 h. Here we present a detailed protocol for this assay and review its applications for studying the immune response to HIV.

UR - http://www.scopus.com/inward/record.url?scp=76649115901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76649115901&partnerID=8YFLogxK

U2 - 10.1038/nprot.2009.227

DO - 10.1038/nprot.2009.227

M3 - Article

C2 - 20134424

AN - SCOPUS:76649115901

VL - 5

SP - 239

EP - 246

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 2

ER -