Synchronization of murine erythroleukemic cells. Nuclear volume measurements for monitoring cell cycle traverse

R. M. Zucker, N. C. Wu, A. Krishan, M. Silverman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Murine erythroleukemic cells (T3C12 clone) were synchronized either by using two 8-h thymidine (2 mM) blocks separated by a 7-h release period, or by centrifugal elutriation. To monitor the synchrony induction and cell cycle traverse, the cells were lysed with NP40 for 20 min and the resulting nuclei fixed with glutaraldehyde. These nuclear volume measurements were performed with a Coulter Electronics H4 cell volume spectrometer and compared with flow cytometric determinations of nuclear DNA content on a Coulter Electronics TPS-1 cell sorter. The results indicate that the nuclear volume measurements can be used as a simple precise method to monitor the cell cycle position and traverse of synchronized erythroleukemic cells.

Original languageEnglish
Pages (from-to)383-387
Number of pages5
JournalExperimental Cell Research
Volume123
Issue number2
DOIs
StatePublished - Oct 15 1979
Externally publishedYes

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Cell Cycle
Glutaral
Cell Size
Thymidine
Clone Cells
DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Synchronization of murine erythroleukemic cells. Nuclear volume measurements for monitoring cell cycle traverse. / Zucker, R. M.; Wu, N. C.; Krishan, A.; Silverman, M.

In: Experimental Cell Research, Vol. 123, No. 2, 15.10.1979, p. 383-387.

Research output: Contribution to journalArticle

Zucker, R. M. ; Wu, N. C. ; Krishan, A. ; Silverman, M. / Synchronization of murine erythroleukemic cells. Nuclear volume measurements for monitoring cell cycle traverse. In: Experimental Cell Research. 1979 ; Vol. 123, No. 2. pp. 383-387.
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