Abstract
Murine erythroleukemic cells (T3C12 clone) were synchronized either by using two 8-h thymidine (2 mM) blocks separated by a 7-h release period, or by centrifugal elutriation. To monitor the synchrony induction and cell cycle traverse, the cells were lysed with NP40 for 20 min and the resulting nuclei fixed with glutaraldehyde. These nuclear volume measurements were performed with a Coulter Electronics H4 cell volume spectrometer and compared with flow cytometric determinations of nuclear DNA content on a Coulter Electronics TPS-1 cell sorter. The results indicate that the nuclear volume measurements can be used as a simple precise method to monitor the cell cycle position and traverse of synchronized erythroleukemic cells.
| Original language | English |
|---|---|
| Pages (from-to) | 383-387 |
| Number of pages | 5 |
| Journal | Experimental Cell Research |
| Volume | 123 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 15 1979 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
Synchronization of murine erythroleukemic cells. Nuclear volume measurements for monitoring cell cycle traverse. / Zucker, R. M.; Wu, N. C.; Krishan, A.; Silverman, M.
In: Experimental Cell Research, Vol. 123, No. 2, 15.10.1979, p. 383-387.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synchronization of murine erythroleukemic cells. Nuclear volume measurements for monitoring cell cycle traverse
AU - Zucker, R. M.
AU - Wu, N. C.
AU - Krishan, A.
AU - Silverman, M.
PY - 1979/10/15
Y1 - 1979/10/15
N2 - Murine erythroleukemic cells (T3C12 clone) were synchronized either by using two 8-h thymidine (2 mM) blocks separated by a 7-h release period, or by centrifugal elutriation. To monitor the synchrony induction and cell cycle traverse, the cells were lysed with NP40 for 20 min and the resulting nuclei fixed with glutaraldehyde. These nuclear volume measurements were performed with a Coulter Electronics H4 cell volume spectrometer and compared with flow cytometric determinations of nuclear DNA content on a Coulter Electronics TPS-1 cell sorter. The results indicate that the nuclear volume measurements can be used as a simple precise method to monitor the cell cycle position and traverse of synchronized erythroleukemic cells.
AB - Murine erythroleukemic cells (T3C12 clone) were synchronized either by using two 8-h thymidine (2 mM) blocks separated by a 7-h release period, or by centrifugal elutriation. To monitor the synchrony induction and cell cycle traverse, the cells were lysed with NP40 for 20 min and the resulting nuclei fixed with glutaraldehyde. These nuclear volume measurements were performed with a Coulter Electronics H4 cell volume spectrometer and compared with flow cytometric determinations of nuclear DNA content on a Coulter Electronics TPS-1 cell sorter. The results indicate that the nuclear volume measurements can be used as a simple precise method to monitor the cell cycle position and traverse of synchronized erythroleukemic cells.
UR - http://www.scopus.com/inward/record.url?scp=0018593920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018593920&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(79)90482-8
DO - 10.1016/0014-4827(79)90482-8
M3 - Article
C2 - 291510
AN - SCOPUS:0018593920
VL - 123
SP - 383
EP - 387
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -