Synaptosomal calcium influx is activated by sodium fluoride

Richard S Jope, Kathleen M. Lally

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Neuronal calcium channels can be modulated by changes in membrane potential or by activation of channel-associated receptors. The latter may be modulated by guanine nucleotide binding proteins. NaF, which activates guanine nucleotide binding proteins, caused a large stimulation of 45Ca2+ uptake by synaptosomes prepared from rat brain. Stimulation of 45Ca2+ influx by NaF (i) was apparent in media containing either 5 mM-K+ or 50 mM-K+, (ii) was slower than the fast-phase of voltage-dependent 45Ca2+ influx but continued for a longer period of time than did depolarization-induced 45Ca2+ influx, and (iii) was not mimicked or modified by a number of drugs, including ouabain, dinitrophenol, sodium azide or sodium vanadate. These results are consistent with the hypothesis that NaF activates a guanine nucleotide binding protein associated with receptor-coupled calcium channels, resulting in stimulation of calcium influx.

Original languageEnglish
Pages (from-to)774-780
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume151
Issue number2
DOIs
StatePublished - Mar 15 1988
Externally publishedYes

Fingerprint

Sodium Fluoride
Guanine Nucleotides
Carrier Proteins
Calcium Channels
Calcium
Dinitrophenols
Sodium Azide
Vanadates
Synaptosomes
Depolarization
Ouabain
Membrane Potentials
Rats
Brain
Chemical activation
Membranes
Electric potential
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synaptosomal calcium influx is activated by sodium fluoride. / Jope, Richard S; Lally, Kathleen M.

In: Biochemical and Biophysical Research Communications, Vol. 151, No. 2, 15.03.1988, p. 774-780.

Research output: Contribution to journalArticle

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