Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling.

Katsuhiko Asanuma, Etsuko Yanagida-Asanuma, Christian H Faul, Yasuhiko Tomino, Kwanghee Kim, Peter Mundel

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

The Rho family of small GTPases (RhoA, Rac1 and Cdc42) controls signal-transduction pathways that influence many aspects of cell behaviour, including cytoskeletal dynamics. At the leading edge, Rac1 and Cdc42 promote cell motility through the formation of lamellipodia and filopodia, respectively. On the contrary, RhoA promotes the formation of contractile actin-myosin-containing stress fibres in the cell body and at the rear. Here, we identify synaptopodin, an actin-associated protein, as a novel regulator of RhoA signalling and cell migration in kidney podocytes. We show that synaptopodin induces stress fibres by competitive blocking of Smurf1-mediated ubiquitination of RhoA, thereby preventing the targeting of RhoA for proteasomal degradation. Gene silencing of synaptopodin in kidney podocytes causes the loss of stress fibres and the formation of aberrant non-polarized filopodia and impairment of cell migration. Together, these data show that synaptopodin is essential for the integrity of the podocyte actin cytoskeleton and for the regulation of podocyte cell migration.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalNature cell biology.
Volume8
Issue number5
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Podocytes
Stress Fibers
Pseudopodia
Cell Movement
Actins
Kidney
Monomeric GTP-Binding Proteins
Ubiquitination
Gene Silencing
Myosins
Actin Cytoskeleton
Signal Transduction
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Asanuma, K., Yanagida-Asanuma, E., Faul, C. H., Tomino, Y., Kim, K., & Mundel, P. (2006). Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling. Nature cell biology., 8(5), 485-491.

Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling. / Asanuma, Katsuhiko; Yanagida-Asanuma, Etsuko; Faul, Christian H; Tomino, Yasuhiko; Kim, Kwanghee; Mundel, Peter.

In: Nature cell biology., Vol. 8, No. 5, 01.05.2006, p. 485-491.

Research output: Contribution to journalArticle

Asanuma, K, Yanagida-Asanuma, E, Faul, CH, Tomino, Y, Kim, K & Mundel, P 2006, 'Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling.', Nature cell biology., vol. 8, no. 5, pp. 485-491.
Asanuma K, Yanagida-Asanuma E, Faul CH, Tomino Y, Kim K, Mundel P. Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling. Nature cell biology. 2006 May 1;8(5):485-491.
Asanuma, Katsuhiko ; Yanagida-Asanuma, Etsuko ; Faul, Christian H ; Tomino, Yasuhiko ; Kim, Kwanghee ; Mundel, Peter. / Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling. In: Nature cell biology. 2006 ; Vol. 8, No. 5. pp. 485-491.
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