Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord

Vivek Patel, Gravil Joseph, Amit Patel, Samik Patel, Devin Bustin, David Mawson, Luis M. Tuesta, Rocio Puentes, Mousumi Ghosh, Damien D Pearse

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwann-cell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

Original languageEnglish
Pages (from-to)789-801
Number of pages13
JournalJournal of Neurotrauma
Volume27
Issue number5
DOIs
StatePublished - May 1 2010

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Schwann Cells
Spinal Cord Injuries
Cell Survival
Spinal Cord
Suspensions
Spinal Cord Regeneration
Laminin
Collagen
Transplants
Therapeutics
Growth

Keywords

  • Collagen
  • Contusion
  • Laminin
  • Matrigel
  • Matrix
  • Methylcellulose

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord. / Patel, Vivek; Joseph, Gravil; Patel, Amit; Patel, Samik; Bustin, Devin; Mawson, David; Tuesta, Luis M.; Puentes, Rocio; Ghosh, Mousumi; Pearse, Damien D.

In: Journal of Neurotrauma, Vol. 27, No. 5, 01.05.2010, p. 789-801.

Research output: Contribution to journalArticle

Patel, V, Joseph, G, Patel, A, Patel, S, Bustin, D, Mawson, D, Tuesta, LM, Puentes, R, Ghosh, M & Pearse, DD 2010, 'Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord', Journal of Neurotrauma, vol. 27, no. 5, pp. 789-801. https://doi.org/10.1089/neu.2008.0809
Patel, Vivek ; Joseph, Gravil ; Patel, Amit ; Patel, Samik ; Bustin, Devin ; Mawson, David ; Tuesta, Luis M. ; Puentes, Rocio ; Ghosh, Mousumi ; Pearse, Damien D. / Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord. In: Journal of Neurotrauma. 2010 ; Vol. 27, No. 5. pp. 789-801.
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