Suspension matrices for improved Schwann-cell survival after implantation into the injured rat spinal cord

Vivek Patel, Gravil Joseph, Amit Patel, Samik Patel, Devin Bustin, David Mawson, Luis M. Tuesta, Rocio Puentes, Mousumi Ghosh, Damien D. Pearse

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwann-cell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

Original languageEnglish (US)
Pages (from-to)789-801
Number of pages13
JournalJournal of neurotrauma
Volume27
Issue number5
DOIs
StatePublished - May 1 2010

Keywords

  • Collagen
  • Contusion
  • Laminin
  • Matrigel
  • Matrix
  • Methylcellulose

ASJC Scopus subject areas

  • Clinical Neurology

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