Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas

Pablo A. Bejarano, Robert P. Baughman, Paul W. Biddinger, Mary Ann Miller, Cecilia Fenoglio-Preiser, Bassim Al-Kafaji, Roberto Di Lauro, Jeffrey A. Whitsett

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

Antibodies to the pulmonary epithelial cell-specific proteins surfactant proteins A and B (SP-A and SP-B) and to thyroid transcription factor-1 (TTF-1), a homeodomain nuclear transcription protein, were used as immunohistochemical markers to asses their ability to distinguish primary pulmonary non-small cell carcinomas (n = 57) from carcinomas of the breast (n = 51). SP-A, SP-B, and ITF-1 were detected in 49%, 53%, and 63% of non-small cell carcinomas, respectively. These three antibodies stained pulmonary adenocarcinomas in 54%, 63% and 76% of specimens, respectively. Squamous cell carcinomas rarely stained using these markers. Antibodies to SP-B and ITF-1 never stained any of the 51 breast carcinomas, whereas four of these tumors stained for SP-A. To better define the potential diagnostic value of these antibodies, 13 breast carcinomas metastatic to the lung were studied. Of the three antibodies tested, only TTF-1 seemed useful, because none of the 13 metastatic tumors showed immunoreactivity to this antibody, whereas six specimens (46%) showed reactivity for both SP-A and SP-B. To emphasize further the potential usefulness of antibodies to TTF-1, sections of adenocarcinomas of the colon (n = 18) and prostate (n = 9), renal cell carcinomas (n = 8), and epithelioid mesotheliomas (n = 4) were evaluated; none was positive. Only one of 66 gastric and one of eight endometrial adenocarcinomas showed focal positivity. These results demonstrate the usefulness of immunodetection of a pulmonary cell selective transcription protein (TTF-1) in the diagnosis of pulmonary adenocarcinoma, readily distinguishing breast carcinomas from primary pulmonary adenocarcinomas. In contrast, staining for SP-A and SP-B is of limited value, because there is an unacceptably high rate of cross-reactivity between breast carcinomas metastatic to the lung and primary pulmonary carcinomas. The latter finding illustrates and supports the fact that tumor marker phenotypes might differ in primary and secondary tissue sites.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalModern Pathology
Volume9
Issue number4
StatePublished - Apr 1 1996

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Surface-Active Agents
Breast Neoplasms
Lung
Antibodies
Proteins
Carcinoma
Adenocarcinoma
Pulmonary Surfactant-Associated Protein A
Homeodomain Proteins
Equidae
Mesothelioma
Tumor Biomarkers
Nuclear Proteins
thyroid nuclear factor 1
Renal Cell Carcinoma
Prostate
Squamous Cell Carcinoma
Neoplasms
Stomach
Colon

Keywords

  • Breast carcinoma
  • Carcinoma
  • Lung
  • Metastasis
  • Surfactant
  • TTF-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bejarano, P. A., Baughman, R. P., Biddinger, P. W., Miller, M. A., Fenoglio-Preiser, C., Al-Kafaji, B., ... Whitsett, J. A. (1996). Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. Modern Pathology, 9(4), 445-452.

Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. / Bejarano, Pablo A.; Baughman, Robert P.; Biddinger, Paul W.; Miller, Mary Ann; Fenoglio-Preiser, Cecilia; Al-Kafaji, Bassim; Di Lauro, Roberto; Whitsett, Jeffrey A.

In: Modern Pathology, Vol. 9, No. 4, 01.04.1996, p. 445-452.

Research output: Contribution to journalArticle

Bejarano, PA, Baughman, RP, Biddinger, PW, Miller, MA, Fenoglio-Preiser, C, Al-Kafaji, B, Di Lauro, R & Whitsett, JA 1996, 'Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas', Modern Pathology, vol. 9, no. 4, pp. 445-452.
Bejarano PA, Baughman RP, Biddinger PW, Miller MA, Fenoglio-Preiser C, Al-Kafaji B et al. Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. Modern Pathology. 1996 Apr 1;9(4):445-452.
Bejarano, Pablo A. ; Baughman, Robert P. ; Biddinger, Paul W. ; Miller, Mary Ann ; Fenoglio-Preiser, Cecilia ; Al-Kafaji, Bassim ; Di Lauro, Roberto ; Whitsett, Jeffrey A. / Surfactant proteins and thyroid transcription factor-1 in pulmonary and breast carcinomas. In: Modern Pathology. 1996 ; Vol. 9, No. 4. pp. 445-452.
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abstract = "Antibodies to the pulmonary epithelial cell-specific proteins surfactant proteins A and B (SP-A and SP-B) and to thyroid transcription factor-1 (TTF-1), a homeodomain nuclear transcription protein, were used as immunohistochemical markers to asses their ability to distinguish primary pulmonary non-small cell carcinomas (n = 57) from carcinomas of the breast (n = 51). SP-A, SP-B, and ITF-1 were detected in 49{\%}, 53{\%}, and 63{\%} of non-small cell carcinomas, respectively. These three antibodies stained pulmonary adenocarcinomas in 54{\%}, 63{\%} and 76{\%} of specimens, respectively. Squamous cell carcinomas rarely stained using these markers. Antibodies to SP-B and ITF-1 never stained any of the 51 breast carcinomas, whereas four of these tumors stained for SP-A. To better define the potential diagnostic value of these antibodies, 13 breast carcinomas metastatic to the lung were studied. Of the three antibodies tested, only TTF-1 seemed useful, because none of the 13 metastatic tumors showed immunoreactivity to this antibody, whereas six specimens (46{\%}) showed reactivity for both SP-A and SP-B. To emphasize further the potential usefulness of antibodies to TTF-1, sections of adenocarcinomas of the colon (n = 18) and prostate (n = 9), renal cell carcinomas (n = 8), and epithelioid mesotheliomas (n = 4) were evaluated; none was positive. Only one of 66 gastric and one of eight endometrial adenocarcinomas showed focal positivity. These results demonstrate the usefulness of immunodetection of a pulmonary cell selective transcription protein (TTF-1) in the diagnosis of pulmonary adenocarcinoma, readily distinguishing breast carcinomas from primary pulmonary adenocarcinomas. In contrast, staining for SP-A and SP-B is of limited value, because there is an unacceptably high rate of cross-reactivity between breast carcinomas metastatic to the lung and primary pulmonary carcinomas. The latter finding illustrates and supports the fact that tumor marker phenotypes might differ in primary and secondary tissue sites.",
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