Abstract
Purpose. To assess intracorneal biocolonization of expanded polytetrafluoroethylene (e-PTFE) materials covalently coated with collagen type-I. Methods. Two e-PTFE membrane types: M-10 (5-30 μm pores, 130 μm thickness, 75% porosity) and Z-30 (30-60 μm pores, 130 μm thickness, 94% porosity) were modified on one side by the covalent attachment of an ultrathin collagen coating. Coated and non-coated disks were inserted in the same right eye cornea of 12 pigmented rabbits, as well as a third e-PTFE type used as a control: 1-80 (40-60 μm pores, 260 μm thickness, 85% porosity). The coated surface of the disks was always facing the anterior side of the cornea. Enucleation was done at 7, 14 days, 1, 3 and 6 months for histology. Results. Both acute and chronic inflammatory response was more pronounced with the collagen coated materials, with neovascularization coming from the limbus towards the membranes anteriorly and a higher density of fibroblastic cells.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Pages | 130-133 |
| Number of pages | 4 |
| Volume | 2971 |
| DOIs | |
| State | Published - 1997 |
| Event | Ophthalmic Technologies VII - San Jose, CA, United States Duration: Feb 8 1997 → Feb 8 1997 |
Other
| Other | Ophthalmic Technologies VII |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 2/8/97 → 2/8/97 |
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Keywords
- Biocolonization
- Collagen
- Cornea
- Gas plasma
- Keratoprosthesis
- Neovascularization
- Porous polytetrafluoroethylene
- Rabbit
- Surface modification
ASJC Scopus subject areas
- Applied Mathematics
- Computer Science Applications
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
Cite this
Surface-modified porous PTFE for keratoprostheses : Biocolonization in the rabbit cornea. / Gautier, Sandrine; Duchesne, Bernard; Tahi, Hassan; Rosa, Robert; Parel, Jean-Marie A; Lacombe, Emmanuel; Beumer, Gerrit; Griesser, Hans J.
Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2971 1997. p. 130-133.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Surface-modified porous PTFE for keratoprostheses
T2 - Biocolonization in the rabbit cornea
AU - Gautier, Sandrine
AU - Duchesne, Bernard
AU - Tahi, Hassan
AU - Rosa, Robert
AU - Parel, Jean-Marie A
AU - Lacombe, Emmanuel
AU - Beumer, Gerrit
AU - Griesser, Hans J.
PY - 1997
Y1 - 1997
N2 - Purpose. To assess intracorneal biocolonization of expanded polytetrafluoroethylene (e-PTFE) materials covalently coated with collagen type-I. Methods. Two e-PTFE membrane types: M-10 (5-30 μm pores, 130 μm thickness, 75% porosity) and Z-30 (30-60 μm pores, 130 μm thickness, 94% porosity) were modified on one side by the covalent attachment of an ultrathin collagen coating. Coated and non-coated disks were inserted in the same right eye cornea of 12 pigmented rabbits, as well as a third e-PTFE type used as a control: 1-80 (40-60 μm pores, 260 μm thickness, 85% porosity). The coated surface of the disks was always facing the anterior side of the cornea. Enucleation was done at 7, 14 days, 1, 3 and 6 months for histology. Results. Both acute and chronic inflammatory response was more pronounced with the collagen coated materials, with neovascularization coming from the limbus towards the membranes anteriorly and a higher density of fibroblastic cells.
AB - Purpose. To assess intracorneal biocolonization of expanded polytetrafluoroethylene (e-PTFE) materials covalently coated with collagen type-I. Methods. Two e-PTFE membrane types: M-10 (5-30 μm pores, 130 μm thickness, 75% porosity) and Z-30 (30-60 μm pores, 130 μm thickness, 94% porosity) were modified on one side by the covalent attachment of an ultrathin collagen coating. Coated and non-coated disks were inserted in the same right eye cornea of 12 pigmented rabbits, as well as a third e-PTFE type used as a control: 1-80 (40-60 μm pores, 260 μm thickness, 85% porosity). The coated surface of the disks was always facing the anterior side of the cornea. Enucleation was done at 7, 14 days, 1, 3 and 6 months for histology. Results. Both acute and chronic inflammatory response was more pronounced with the collagen coated materials, with neovascularization coming from the limbus towards the membranes anteriorly and a higher density of fibroblastic cells.
KW - Biocolonization
KW - Collagen
KW - Cornea
KW - Gas plasma
KW - Keratoprosthesis
KW - Neovascularization
KW - Porous polytetrafluoroethylene
KW - Rabbit
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=58049203984&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58049203984&partnerID=8YFLogxK
U2 - 10.1117/12.275104
DO - 10.1117/12.275104
M3 - Conference contribution
VL - 2971
SP - 130
EP - 133
BT - Proceedings of SPIE - The International Society for Optical Engineering
ER -