Surface chemistry and spectroscopy of the β-galactosidase Langmuir monolayer

Nicholas F. Crawford, Miodrag Micic, Jhony Orbulescu, Daniel Weissbart, Roger Leblanc

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The changes of interfacial properties of β-galactosidase introduced into different pH environments are investigated through surface chemistry and in situ spectroscopy. Conditions for an optimal Langmuir monolayer formation were firstly obtained by varying the subphase salt concentration and the surface-pressure area isotherm was used to extrapolate the limiting molecular area of the enzyme monolayer to be around 42,000Å2molecule-1. Surface pressure stability measurements held at 20mN/m for 90min along with compression-decompression cycles revealed no aggregate formation at the air-water interface. Consistent with the data obtained from the isotherm, in situ UV-Vis and fluorescence spectroscopy shows a steep rise in absorbance and photoluminescence intensity correlating to with a switch from a liquid-expanded to a liquid-condensed phase. A decrease in subphase pH increased the electrostatic repulsion as the enzyme was protonated, leading to an expanded monolayer. Infrared absorption-reflection spectroscopy demonstrates that the enzyme adopts mainly β-sheet conformation at the air-water interface before and during the compression.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalJournal of Colloid and Interface Science
Volume453
DOIs
StatePublished - Sep 1 2015

Keywords

  • Langmuir monolayer
  • Photophysical properties
  • Spectroscopy
  • β-Galactosidase

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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