Abstract
Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of die subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.
| Original language | English |
|---|---|
| Pages (from-to) | 4888-4893 |
| Number of pages | 6 |
| Journal | Langmuir |
| Volume | 24 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 6 2008 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
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Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer. / Thakur, Garima; Wang, Chengshan; Leblanc, Roger.
In: Langmuir, Vol. 24, No. 9, 06.05.2008, p. 4888-4893.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer
AU - Thakur, Garima
AU - Wang, Chengshan
AU - Leblanc, Roger
PY - 2008/5/6
Y1 - 2008/5/6
N2 - Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of die subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.
AB - Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of die subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.
UR - http://www.scopus.com/inward/record.url?scp=44249116931&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44249116931&partnerID=8YFLogxK
U2 - 10.1021/la703893m
DO - 10.1021/la703893m
M3 - Article
C2 - 18355099
AN - SCOPUS:44249116931
VL - 24
SP - 4888
EP - 4893
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 9
ER -