Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer

Garima Thakur, Chengshan Wang, Roger Leblanc

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of die subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.

Original languageEnglish
Pages (from-to)4888-4893
Number of pages6
JournalLangmuir
Volume24
Issue number9
DOIs
StatePublished - May 6 2008

Fingerprint

monomolecular films
lysozyme
Muramidase
Surface chemistry
Monolayers
Enzymes
Spectroscopy
chemistry
spectroscopy
homogeneity
pressure reduction
chromophores
Fluorescence spectroscopy
Surface potential
Chromophores
isotherms
Ultraviolet spectroscopy
Isotherms
salts
fluorescence

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer. / Thakur, Garima; Wang, Chengshan; Leblanc, Roger.

In: Langmuir, Vol. 24, No. 9, 06.05.2008, p. 4888-4893.

Research output: Contribution to journalArticle

Thakur, Garima ; Wang, Chengshan ; Leblanc, Roger. / Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer. In: Langmuir. 2008 ; Vol. 24, No. 9. pp. 4888-4893.
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