Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer

Garima Thakur, Chengshan Wang, Roger M. Leblanc

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of die subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.

Original languageEnglish (US)
Pages (from-to)4888-4893
Number of pages6
JournalLangmuir
Volume24
Issue number9
DOIs
StatePublished - May 6 2008

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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