Supramolecular delivery of photoactivatable fluorophores in developing embryos

Yang Zhang, Sicheng Tang, Lorenzo Sansalone, Ek Raj Thapaliya, James Baker, Francisco Raymo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.

Original languageEnglish (US)
Title of host publicationColloidal Nanoparticles for Biomedical Applications XII
PublisherSPIE
Volume10078
ISBN (Electronic)9781510605978
DOIs
StatePublished - 2017
EventColloidal Nanoparticles for Biomedical Applications XII 2017 - San Francisco, United States
Duration: Jan 28 2017Jan 31 2017

Other

OtherColloidal Nanoparticles for Biomedical Applications XII 2017
CountryUnited States
CitySan Francisco
Period1/28/171/31/17

Fingerprint

Fluorophores
embryos
delivery
Embryonic Structures
Fluorescence
Blastoderm
Drosophila
Biological Phenomena
fluorescence
Drosophila melanogaster
Fluorescent Dyes
organisms
switches
Switches
Irradiation
Light
Molecules
irradiation
probes
products

Keywords

  • Drosophila Melanogaster
  • fluorescence imaging
  • molecular switches
  • nanoparticles
  • Photoactivation
  • self-assembly

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Zhang, Y., Tang, S., Sansalone, L., Thapaliya, E. R., Baker, J., & Raymo, F. (2017). Supramolecular delivery of photoactivatable fluorophores in developing embryos. In Colloidal Nanoparticles for Biomedical Applications XII (Vol. 10078). [100781A] SPIE. https://doi.org/10.1117/12.2261721

Supramolecular delivery of photoactivatable fluorophores in developing embryos. / Zhang, Yang; Tang, Sicheng; Sansalone, Lorenzo; Thapaliya, Ek Raj; Baker, James; Raymo, Francisco.

Colloidal Nanoparticles for Biomedical Applications XII. Vol. 10078 SPIE, 2017. 100781A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, Y, Tang, S, Sansalone, L, Thapaliya, ER, Baker, J & Raymo, F 2017, Supramolecular delivery of photoactivatable fluorophores in developing embryos. in Colloidal Nanoparticles for Biomedical Applications XII. vol. 10078, 100781A, SPIE, Colloidal Nanoparticles for Biomedical Applications XII 2017, San Francisco, United States, 1/28/17. https://doi.org/10.1117/12.2261721
Zhang Y, Tang S, Sansalone L, Thapaliya ER, Baker J, Raymo F. Supramolecular delivery of photoactivatable fluorophores in developing embryos. In Colloidal Nanoparticles for Biomedical Applications XII. Vol. 10078. SPIE. 2017. 100781A https://doi.org/10.1117/12.2261721
Zhang, Yang ; Tang, Sicheng ; Sansalone, Lorenzo ; Thapaliya, Ek Raj ; Baker, James ; Raymo, Francisco. / Supramolecular delivery of photoactivatable fluorophores in developing embryos. Colloidal Nanoparticles for Biomedical Applications XII. Vol. 10078 SPIE, 2017.
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