Suppression of meiosis of male germ cells by an antagonist of luteinizing hormone-releasing hormone

Bela Szende, Tommie W. Redding, Andrew V. Schally

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Male nude mice were implanted with osmotic minipumps releasing 50 μg of a potent antagonist of luteinizing hormone-releasing hormone (LH-RH) per day {N-Ac-[D-Nal(2)1,D-Phe(pCl)2,D-Pal(3) 3,D-Cit6,D-Ala10]LH-RH} (SB-75) [Nal(2), 3-(2-naphthyl)alanine; Phe(pCl), 4-chlorophenyl-alanine; Pal(3), 3-(3-pyridyl)alanine; Cit, citrulline], or they were treated with s.c. injections of SB-75 (25 μg twice a day). Another group of nude mice received an injection of micro-capsules of the agonist [D-Trp6]LH-RH liberating 25 μg/day. One month after the initiation of treatment, the testicular weights were significantly reduced and the blood testosterone values were at castration levels in all treated groups. Histologically, only the testicles of the mice treated with SB-75 released from minipumps showed a significant decrease of meiosis. The most advanced forms of germ cells were spermatogonia in 26 %, spermatocytes in 17%, and round spermatids in 35% of the seminiferous tubules. Only 22% of the tubules contained elongated spermatids. The suppression of meiotic activity by this modern LH-RH antagonist can possibly be used for the development of methods for male contraception and for the protection of germ cells against the damage caused by cytotoxic drugs and x-radiation.

Original languageEnglish (US)
Pages (from-to)901-903
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number3
DOIs
StatePublished - 1990

Keywords

  • Analogs of luteinizing hormone-releasing hormone
  • Inhibition of spermatogenesis

ASJC Scopus subject areas

  • Genetics
  • General

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