Suppression of human IgE antibody forming cell responses by IL-6

G. I. Kleiner, D. L. Auci, A. Shaikh, S. M. Chice, H. G. Durkin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

To study the effects of cytokines on human IgE antibody forming cells (AFCs), log phase U266 myeloma cells (3 x 103/ml), which secrete immunoglobulin E (IgE), were cultured for 0-24 h with and without cytokine or with or without antibodies against various cytokines. The numbers of IgE AFCs were determined in ELISPOT assay. We found that interleukin-6 (IL-6) suppressed (to 95%) whereas anti-IL-6 increased (to 148%) the numbers of IgE AFCs and that both worked in a dose-dependent fashion. IL-4 and interferon- γ (IFN-γ) also suppressed IgE AFC responses in a dose-dependent fashion. However, antibodies to these cytokines had no effect. In contrast, IFN-α increased (to fourfold) the numbers of IgE AFCs in a dose-dependent fashion. The data are the first to show a suppressive effect of IL-6 on human IgE responses and may also suggest a role for IL-6 in the treatment of atopic disease.

Original languageEnglish (US)
Pages (from-to)168-170
Number of pages3
JournalJournal of Leukocyte Biology
Volume54
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • allergy
  • cytokines
  • IgE
  • IL-6

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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