Suppression of clonogenic potential of human bone marrow mesenchymal stem cells by HIV type 1

Putative role of HIV type 1 Tat protein and inflammatory cytokines

Lixin Wang, Debasis Mondal, Vincent F. La Russa, Krishna C. Agrawal

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Bone marrow abnormalities are frequently observed in HIV-1-infected individuals. Infection of marrow mesenchymal stem cells (MSCs) may abrogate their growth properties and hematopoietic supportive functions. To delineate the cell type infected, and factors responsible for the deleterious effects, human bone marrow cells were exposed to HIV-1 in vitro. By week 4, the ability of MSCs to form colonies of purely fibroblasts (CFU-F) and mixed colonies of fibroblasts and adipocytes (CFU-FA) was suppressed by 23 ± 5 and 55 ± 7%, respectively. The p24 concentration in culture supernatants steadily declined from 170 ng/ml in the inoculum to 134 ± 30, 35 ± 15, 2.3 ± 3, and < 0.02 ng/ml at the end of week 1, 2, 3, and 4, respectively. However, even at week 4, coculturing with MT-4 lymphocytes for 1 week dramatically increased p24 levels. Polymerase chain reaction (PCR) amplification, using HIV-1-specific primers, and in situ hybridization with an HIV-1 cDNA probe demonstrated the presence of virus-specific nucleic acids within stromal colonies. Coimmunostaining with antibody to CD83 implicated the presence of HIV-1 within dendritic progenitor cells. Immunostaining with HIV-1 Tat antibody demonstrated the presence of Tat protein and reverse transcriptase (RT)-PCR assays showed increased (160-220%) mRNA levels for inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin 1β [IL-1β], IL-6, and macrophage inflammatory protein 1α [MIP-1α]). A concentration-dependent decrease in CFU-STROs was observed on incubation with either Tat protein (1-100 ng/ml) or with TNF-α or IL-1β (0.025-25 ng/ml). These results suggest that HIV-1 infection of stromal cells may produce inhibitory factors that suppress the clonogenic potential of MSCs.

Original languageEnglish
Pages (from-to)917-931
Number of pages15
JournalAIDS Research and Human Retroviruses
Volume18
Issue number13
DOIs
StatePublished - Oct 12 2002

Fingerprint

Human Immunodeficiency Virus tat Gene Products
Mesenchymal Stromal Cells
HIV-1
Bone Marrow
Cytokines
tat Gene Products
Interleukin-1
Tumor Necrosis Factor-alpha
Fibroblasts
Macrophage Inflammatory Proteins
Antibodies
Stromal Cells
Reverse Transcriptase Polymerase Chain Reaction
Adipocytes
Bone Marrow Cells
Dendritic Cells
Nucleic Acids
HIV Infections
In Situ Hybridization
Interleukin-6

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Suppression of clonogenic potential of human bone marrow mesenchymal stem cells by HIV type 1 : Putative role of HIV type 1 Tat protein and inflammatory cytokines. / Wang, Lixin; Mondal, Debasis; La Russa, Vincent F.; Agrawal, Krishna C.

In: AIDS Research and Human Retroviruses, Vol. 18, No. 13, 12.10.2002, p. 917-931.

Research output: Contribution to journalArticle

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abstract = "Bone marrow abnormalities are frequently observed in HIV-1-infected individuals. Infection of marrow mesenchymal stem cells (MSCs) may abrogate their growth properties and hematopoietic supportive functions. To delineate the cell type infected, and factors responsible for the deleterious effects, human bone marrow cells were exposed to HIV-1 in vitro. By week 4, the ability of MSCs to form colonies of purely fibroblasts (CFU-F) and mixed colonies of fibroblasts and adipocytes (CFU-FA) was suppressed by 23 ± 5 and 55 ± 7{\%}, respectively. The p24 concentration in culture supernatants steadily declined from 170 ng/ml in the inoculum to 134 ± 30, 35 ± 15, 2.3 ± 3, and < 0.02 ng/ml at the end of week 1, 2, 3, and 4, respectively. However, even at week 4, coculturing with MT-4 lymphocytes for 1 week dramatically increased p24 levels. Polymerase chain reaction (PCR) amplification, using HIV-1-specific primers, and in situ hybridization with an HIV-1 cDNA probe demonstrated the presence of virus-specific nucleic acids within stromal colonies. Coimmunostaining with antibody to CD83 implicated the presence of HIV-1 within dendritic progenitor cells. Immunostaining with HIV-1 Tat antibody demonstrated the presence of Tat protein and reverse transcriptase (RT)-PCR assays showed increased (160-220{\%}) mRNA levels for inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin 1β [IL-1β], IL-6, and macrophage inflammatory protein 1α [MIP-1α]). A concentration-dependent decrease in CFU-STROs was observed on incubation with either Tat protein (1-100 ng/ml) or with TNF-α or IL-1β (0.025-25 ng/ml). These results suggest that HIV-1 infection of stromal cells may produce inhibitory factors that suppress the clonogenic potential of MSCs.",
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