Suppression of cell-mediated tumor cell lysis and complement-induced cytotoxicity in trypan blue

J. C. Scornik, P. Ruiz, E. M. Hoffman

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Different forms of cell-mediated cytotoxicity were suppressed in the presence of trypan blue. The systems affected included lysis of antibody-coated tumor cells by normal and C. parvum-stimulated mouse peritoneal cells and lysis of allogeneic targets by immune effector cells. The inhibition, measured in a 4-hr 51Cr release assay, was reversible and did not occur in the presence of 30% fetal calf serum or albumin. Binding between effector and target cells through Fc receptors was not affected, and lysis of allogeneic cells was inhibited at the lytic step rather than at the binding step. In contrast, lysis of sensitized erythrocytes was not inhibited by trypan blue, suggesting that lysis of these targets may not involve the steps required in tumor cell lysis. Trypan blue blocked the function of antibody before binding to target cells and also suppressed complement-induced cytolysis. Most individual complement components were susceptible to the inhibitory action of trypan blue. These results reveal an affinity of trypan blue for protein in general that may be responsible for many of its biologic actions.

Original languageEnglish (US)
Pages (from-to)1278-1284
Number of pages7
JournalJournal of Immunology
Volume123
Issue number3
StatePublished - Jan 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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