Superresolution imaging with switchable fluorophores based on oxazine auxochromes

Marco Petriella, Erhan Deniz, Subramani Swaminathan, Maria J. Roberti, Francisco Raymo, Mariano L. Bossi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The spatial resolution of fluorescence microscopes is limited by diffraction to about half of the light wavelength, hampering the observation of many important intracellular processes. Recent emerging techniques have overcome that diffraction barrier using the temporal discrimination of close objects that are otherwise unresolved or blurred within the spatial resolution of the microscope. The key of these techniques is to switch the signal of fluorescence markers on and off exploiting their distinct molecular states, and detect and localize these markers at the single-molecule level. This underlying principle highlights the critical role of the photophysical properties of the probes, and the importance of finding adequate switching mechanisms. Here, we present strategies to achieve fluorescence modulation based on novel molecular assemblies containing a [1,3]oxazine as the two states, building block responsible for the transformation. Two different triggering events, based on the photochromic and halochromic properties of the oxazine, induce a large absorption and emission bathochromic shift of a pendant fluorophore, as the ultimate fluorescence switching event. The implementation of these approaches to achieve spatial resolution beyond the diffraction limit is also discussed.

Original languageEnglish
Pages (from-to)1391-1398
Number of pages8
JournalPhotochemistry and Photobiology
Volume89
Issue number6
DOIs
StatePublished - Nov 1 2013

Fingerprint

Oxazines
Fluorophores
Fluorescence
Imaging techniques
fluorescence
Diffraction
spatial resolution
markers
Microscopes
microscopes
diffraction
assemblies
discrimination
emerging
Switches
Observation
Modulation
modulation
Light
Wavelength

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Petriella, M., Deniz, E., Swaminathan, S., Roberti, M. J., Raymo, F., & Bossi, M. L. (2013). Superresolution imaging with switchable fluorophores based on oxazine auxochromes. Photochemistry and Photobiology, 89(6), 1391-1398. https://doi.org/10.1111/php.12100

Superresolution imaging with switchable fluorophores based on oxazine auxochromes. / Petriella, Marco; Deniz, Erhan; Swaminathan, Subramani; Roberti, Maria J.; Raymo, Francisco; Bossi, Mariano L.

In: Photochemistry and Photobiology, Vol. 89, No. 6, 01.11.2013, p. 1391-1398.

Research output: Contribution to journalArticle

Petriella, M, Deniz, E, Swaminathan, S, Roberti, MJ, Raymo, F & Bossi, ML 2013, 'Superresolution imaging with switchable fluorophores based on oxazine auxochromes', Photochemistry and Photobiology, vol. 89, no. 6, pp. 1391-1398. https://doi.org/10.1111/php.12100
Petriella, Marco ; Deniz, Erhan ; Swaminathan, Subramani ; Roberti, Maria J. ; Raymo, Francisco ; Bossi, Mariano L. / Superresolution imaging with switchable fluorophores based on oxazine auxochromes. In: Photochemistry and Photobiology. 2013 ; Vol. 89, No. 6. pp. 1391-1398.
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