TY - JOUR
T1 - Superantigen-Mediated Deletion of Specific T Cell Receptor Vβ Subsets in the SCID-hu Thy/ Liv Mouse Is Induced by Staphylococcal Enterotoxin B, but Not HIV-I
AU - Komanduri, Krishna V.
AU - Salha, Marie Danielle
AU - Sékaly, Rafick Pierre
AU - McCune, Joseph M.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - Infection by HIV-1 has been associated with perturbations in the TCR Vβ repertoire, suggesting the involvement of a superantigen. Among the hallmarks of superantigens is the capacity to delete T cells bearing specific TCR Vβ families in the developing thymus. To verify the presence of a superantigen in HIV-1, we analyzed the SCID-hu Thy/Liv TCR Vβ repertoire within CD4+CD8+, CD4+CD8-, or CD4-CD8+ thymocytes subsets by flow cytometry using a panel of Abs recognizing about 60% of the TCR repertoire following injection of SEB or infection by two different HIV-1 isolates. Seven days following SEB injection, thymocyte subsets bearing TCR Vβ3, Vβ12, Vβ17, and Vβ20, but not Vβ5 or Vβ8, were deleted relative to mock-injected mice. In contrast, no changes were observed in the TCR Vβ repertoire in CD4+CD8+, CD4+CD8-, or CD4-CD8+ thymocyte subsets after infection with HIV-1. The T cell depletion caused by HIV-1 infection is most likely not mediated by an HIV-encoded superantigen.
AB - Infection by HIV-1 has been associated with perturbations in the TCR Vβ repertoire, suggesting the involvement of a superantigen. Among the hallmarks of superantigens is the capacity to delete T cells bearing specific TCR Vβ families in the developing thymus. To verify the presence of a superantigen in HIV-1, we analyzed the SCID-hu Thy/Liv TCR Vβ repertoire within CD4+CD8+, CD4+CD8-, or CD4-CD8+ thymocytes subsets by flow cytometry using a panel of Abs recognizing about 60% of the TCR repertoire following injection of SEB or infection by two different HIV-1 isolates. Seven days following SEB injection, thymocyte subsets bearing TCR Vβ3, Vβ12, Vβ17, and Vβ20, but not Vβ5 or Vβ8, were deleted relative to mock-injected mice. In contrast, no changes were observed in the TCR Vβ repertoire in CD4+CD8+, CD4+CD8-, or CD4-CD8+ thymocyte subsets after infection with HIV-1. The T cell depletion caused by HIV-1 infection is most likely not mediated by an HIV-encoded superantigen.
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M3 - Article
C2 - 8992966
AN - SCOPUS:0031568082
VL - 158
SP - 544
EP - 549
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -