Abstract
Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-96 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 287 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Aug 5 1991 |
| Externally published | Yes |
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Keywords
- Cell proliferation
- Immune
- Interleukin
- Opioid binding
- Phorbol ester
- Up-regulation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
[3H]Morphine binding is enhanced by IL-1-stimulated thymocyte proliferation. / Roy, Sabita; Ge, Bang Lun; Ramakrishnan, Sundaram; Lee, Nancy M.; Loh, Horace H.
In: FEBS Letters, Vol. 287, No. 1-2, 05.08.1991, p. 93-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - [3H]Morphine binding is enhanced by IL-1-stimulated thymocyte proliferation
AU - Roy, Sabita
AU - Ge, Bang Lun
AU - Ramakrishnan, Sundaram
AU - Lee, Nancy M.
AU - Loh, Horace H.
PY - 1991/8/5
Y1 - 1991/8/5
N2 - Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.
AB - Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.
KW - Cell proliferation
KW - Immune
KW - Interleukin
KW - Opioid binding
KW - Phorbol ester
KW - Up-regulation
UR - http://www.scopus.com/inward/record.url?scp=0025923622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025923622&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(91)80023-V
DO - 10.1016/0014-5793(91)80023-V
M3 - Article
C2 - 1652466
AN - SCOPUS:0025923622
VL - 287
SP - 93
EP - 96
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -