Abstract
Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.
Original language | English (US) |
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Pages (from-to) | 93-96 |
Number of pages | 4 |
Journal | FEBS letters |
Volume | 287 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 5 1991 |
Externally published | Yes |
Keywords
- Cell proliferation
- Immune
- Interleukin
- Opioid binding
- Phorbol ester
- Up-regulation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology