3H-serotonin as a marker of oscillatory insulin secretion in clonal β-cells (INS-1)

Jude T. Deeney, Robert Bränström, Barbara E. Corkey, Olof Larsson, Per Olof Berggren

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Serotonin release from preloaded pancreatic β-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic β-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.

Original languageEnglish (US)
Pages (from-to)4080-4084
Number of pages5
JournalFEBS letters
Volume581
Issue number21
DOIs
StatePublished - Aug 21 2007

Keywords

  • Exocytosis
  • Insulin secretion
  • Insulin-secreting cell
  • Serotonin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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