31P-Nuclear Magnetic Resonance Studies of the Effect of Recombinant Human Interleukin la on the Bioenergetics of RIF-1 Tumors

Ioannis Constantinidis, Janna P. Wehrle, Jerry D. Glickson, Paul G. Braunschweiger, Nirmal Kumar, Candace S. Johnson, Philip Furmanski

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The effect of a single injection of human recombinant interleukin 1α (IL-1α) on s.c. RIF-1 tumors in mice was studied by in vivo 31P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-1α. At 2, 4, 6, and 8 h after IL-1α injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P(i) to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P(i) and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-1α treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-1α treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-1α. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.

Original languageEnglish (US)
Pages (from-to)6379-6382
Number of pages4
JournalCancer Research
Volume49
Issue number22
StatePublished - Nov 15 1989

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of '31P-Nuclear Magnetic Resonance Studies of the Effect of Recombinant Human Interleukin la on the Bioenergetics of RIF-1 Tumors'. Together they form a unique fingerprint.

  • Cite this

    Constantinidis, I., Wehrle, J. P., Glickson, J. D., Braunschweiger, P. G., Kumar, N., Johnson, C. S., & Furmanski, P. (1989). 31P-Nuclear Magnetic Resonance Studies of the Effect of Recombinant Human Interleukin la on the Bioenergetics of RIF-1 Tumors. Cancer Research, 49(22), 6379-6382.