31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors

I. Constantinidis, P. G. Braunschweiger, J. P. Wehrle, Paul G Braunschweiger, C. S. Johnson, P. Furmanski, J. D. Glickson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effect of a single injection of human recombinant interleukin 1α (IL-1α) on s.c. RIF-1 tumors in mice was studied by in vivo 31P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-1α. At 2, 4, 6, and 8 h after IL-1α injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P(i) to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P(i) and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-1α treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-1α treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-1α. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.

Original languageEnglish
Pages (from-to)6379-6382
Number of pages4
JournalCancer Research
Volume49
Issue number22
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Interleukin-1
Energy Metabolism
Magnetic Resonance Spectroscopy
Neoplasms
Nucleosides
Injections
Phosphocreatine
Necrosis
Therapeutics
Perfusion
Hemorrhage

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Constantinidis, I., Braunschweiger, P. G., Wehrle, J. P., Braunschweiger, P. G., Johnson, C. S., Furmanski, P., & Glickson, J. D. (1989). 31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors. Cancer Research, 49(22), 6379-6382.

31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors. / Constantinidis, I.; Braunschweiger, P. G.; Wehrle, J. P.; Braunschweiger, Paul G; Johnson, C. S.; Furmanski, P.; Glickson, J. D.

In: Cancer Research, Vol. 49, No. 22, 01.01.1989, p. 6379-6382.

Research output: Contribution to journalArticle

Constantinidis, I, Braunschweiger, PG, Wehrle, JP, Braunschweiger, PG, Johnson, CS, Furmanski, P & Glickson, JD 1989, '31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors', Cancer Research, vol. 49, no. 22, pp. 6379-6382.
Constantinidis I, Braunschweiger PG, Wehrle JP, Braunschweiger PG, Johnson CS, Furmanski P et al. 31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors. Cancer Research. 1989 Jan 1;49(22):6379-6382.
Constantinidis, I. ; Braunschweiger, P. G. ; Wehrle, J. P. ; Braunschweiger, Paul G ; Johnson, C. S. ; Furmanski, P. ; Glickson, J. D. / 31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors. In: Cancer Research. 1989 ; Vol. 49, No. 22. pp. 6379-6382.
@article{585876bef1de4342872a8368dada7102,
title = "31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors",
abstract = "The effect of a single injection of human recombinant interleukin 1α (IL-1α) on s.c. RIF-1 tumors in mice was studied by in vivo 31P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-1α. At 2, 4, 6, and 8 h after IL-1α injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P(i) to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P(i) and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-1α treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-1α treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-1α. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.",
author = "I. Constantinidis and Braunschweiger, {P. G.} and Wehrle, {J. P.} and Braunschweiger, {Paul G} and Johnson, {C. S.} and P. Furmanski and Glickson, {J. D.}",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "49",
pages = "6379--6382",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "22",

}

TY - JOUR

T1 - 31P-nuclear magnetic resonance studies of the effect of recombinant human interleukin 1α on the bioenergetics of RIF-1 tumors

AU - Constantinidis, I.

AU - Braunschweiger, P. G.

AU - Wehrle, J. P.

AU - Braunschweiger, Paul G

AU - Johnson, C. S.

AU - Furmanski, P.

AU - Glickson, J. D.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The effect of a single injection of human recombinant interleukin 1α (IL-1α) on s.c. RIF-1 tumors in mice was studied by in vivo 31P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-1α. At 2, 4, 6, and 8 h after IL-1α injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P(i) to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P(i) and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-1α treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-1α treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-1α. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.

AB - The effect of a single injection of human recombinant interleukin 1α (IL-1α) on s.c. RIF-1 tumors in mice was studied by in vivo 31P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-1α. At 2, 4, 6, and 8 h after IL-1α injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P(i) to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P(i) and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-1α treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-1α treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-1α. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.

UR - http://www.scopus.com/inward/record.url?scp=0024473457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024473457&partnerID=8YFLogxK

M3 - Article

C2 - 2804984

AN - SCOPUS:0024473457

VL - 49

SP - 6379

EP - 6382

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 22

ER -