1H NMR studies distinguish the water soluble metabolomic profiles of untransformed and RAS-transformed cells

Vered Marks, Anisleidys Munoz, Priyamvada Rai, Jamie D. Walls

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Metabolomic profiling is an increasingly important method for identifying potential biomarkers in cancer cells with a view towards improved diagnosis and treatment. Nuclear magnetic resonance (NMR) provides a potentially noninvasive means to accurately characterize differences in the metabolomic profiles of cells. In this work, we use 1HNMRto measure the metabolomic profiles of water soluble metabolites extracted from isogenic control and oncogenic HRAS-, KRAS-, and NRAS-transduced BEAS2B lung epithelial cells to determine the robustness of NMR metabolomic profiling in detecting differences between the transformed cells and their untransformed counter- parts as well as differences among the RAS-transformed cells. Unique metabolomic signatures between control and RAS-transformed cell lines as well as among the three RAS isoform-transformed lines were found by applying principal component analysis to theNMRdata. This study provides a proof of principle demonstration that NMR-based metabolomic profiling can robustly distinguish untransformed and RAS-transformed cells as well as cells transformed with different RAS oncogenic isoforms. Thus, our data may potentially provide new diagnostic signatures for RAS-transformed cells.

Original languageEnglish (US)
Article numbere2104
JournalPeerJ
Volume2016
Issue number6
DOIs
StatePublished - 2016

Keywords

  • 1D NOESY
  • Fieller's method for unpaired data
  • H NMR metabolomics
  • Oncogenic RAS

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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