Sulfite inhibition of photochemical activity of intact pea leaves

Konka Veeranjaneyulu, Denis Charlebois, Christophe N. N'soukpoé-Kossi, Roger M. Leblanc

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Sulfite treatment of pea leaf disks in light caused a significant decrease in the relative quantum yield of photosynthetic oxygen evolution and energy storage (ES) as measured by photoacoustic (PA) spectroscopy. The inhibition was concentration dependent and was less in darkness than in light, indicating light-dependent inhibitory site(s) on the photosynthetic electron transport chain. Further, in darksulfite-treated leaves, the energy storage was more affected than the relative quantum yield of oxygen evolution, suggesting that photophosphorylation and/or cyclic electron transport around PS I are sites of sulfite action in darkness. The Rfd values, the ratio of fluorescence decrease (fd) to the steady-state fluorescence (fs), decreased significantly in leaves treated with sulfite in light but were not affected in dark-treated ones, confirming the photoacoustic observations. Similarly, the ratio of variable fluorescence (Fv) to maximum fluorescence (Fm), a measure of PS II photochemical efficiency, was affected by sulfite treatment in light and not changed by treatment in darkness. An attempt was made to explain the mechanism of sulfite action on photosynthetic electron transport in light and in darkness.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalPhotosynthesis Research
Volume34
Issue number2
DOIs
StatePublished - Nov 1 1992
Externally publishedYes

Keywords

  • energy storage
  • oxygen evolution
  • photoacoustic spectroscopy

ASJC Scopus subject areas

  • Plant Science

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