Successful application of hyperbranched multidisplacement genomic amplification to detect HIV-1 sequences in single neurons removed from autopsy brain sections by laser capture microdissection

Jorge E. Torres-Muñoz, Mariana Núñez, Carol K. Petito

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

To confirm studies suggesting that HIV-1 infects neurons and to determine whether CD8+ T lymphocytes traffic to HIV-1-infected neurons, we used laser capture microdissection to remove hippocampal neurons with and without perineuronal CD8+ T cells from AIDS patients with HIV-1 encephalitis (HIVE) or without HIVE and from normal controls. We used hyperbranched multidisplacement amplification for whole gene amplification (MDA-WGA) plus two rounds of PCR to amplify housekeeping sequences (HK+) and, in HK + samples, to amplify HIV-1 gag, nef, and pol sequences. Sample size and, in single neurons, MDA-WGA correlated with housekeeping gene amplification (P < 0.05), whereas patient group and postmortem interval did not (P > 0.05). Neuronal viral sequences correlated with HIVE (43% vs. 13% and 0 in non-HIVE and controls, respectively) and, in HIVE cases, with perlneuronal CD8+ T lymphocytes (70% in CD8+ samples vs. 37% of CD8- samples). Our results suggest that MDA-WGA is a useful technique when analyzing DNA from single cells from autopsy brains, supporting prior studies that show that neurons may contain HIV-1 neuronal sequences in vivo. The association between neuronal infection and perineuronal CD8+ T cells supports our hypothesis that these cells specifically traffic to infected neurons but raises the possibility that CD8+ T cells, if infected, could transmit virus to neurons.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalJournal of Molecular Diagnostics
Volume10
Issue number4
DOIs
StatePublished - Jul 2008

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine

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