Subunit contributions to insect olfactory receptor function: Channel block and odorant recognition

Andrew S. Nichols, Sisi Chen, Charles W Luetje

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Insect olfactory receptors are heteromeric ligand-gated ion channels composed of at least one common subunit (Orco) and at least one subunit that confers odorant specificity. Little is known about how individual subunits contribute to the structure and function of the olfactory receptor complex. We expressed insect olfactory receptors in Xenopus oocytes to investigate 2 functional features, ion channel block and odorant recognition. The sensitivity of Drosophila olfactory receptors to inhibition by ruthenium red, a cation channel blocker, varied widely when different specificity subunits were present, suggesting that the specificity subunits contribute to the structure of the ion pore. Olfactory receptors formed by Dmel\Or35a and Orco subunits from several different species displayed highly similar odorant response profiles, suggesting that the Orco subunit does not contribute to the structure of the odorant-binding site. We further explored odorant recognition by conducting a detailed examination of the odorant specificity Dmel\Or67a + Dmel\Orco, a receptor that responds to aromatic structures. This screen identified agonists, partial agonists, and an antagonist of Dmel\Or67a + Dmel\Orco. Our findings favor specific subunit arrangements within the olfactory receptor complex and provide a preliminary odorophore for an olfactory receptor, offering a useful foundation for future exploration of insect olfactory receptor structure.

Original languageEnglish
Pages (from-to)781-790
Number of pages10
JournalChemical Senses
Volume36
Issue number9
DOIs
StatePublished - Nov 1 2011

Fingerprint

Odorant Receptors
Insects
Ligand-Gated Ion Channels
Ruthenium Red
Odorants
Xenopus
Ion Channels
Drosophila
Oocytes
Cations
Binding Sites
Ions

Keywords

  • Electrophysiology
  • Insect olfactory receptors
  • Xenopus oocytes

ASJC Scopus subject areas

  • Sensory Systems
  • Behavioral Neuroscience
  • Physiology
  • Physiology (medical)

Cite this

Subunit contributions to insect olfactory receptor function : Channel block and odorant recognition. / Nichols, Andrew S.; Chen, Sisi; Luetje, Charles W.

In: Chemical Senses, Vol. 36, No. 9, 01.11.2011, p. 781-790.

Research output: Contribution to journalArticle

Nichols, Andrew S. ; Chen, Sisi ; Luetje, Charles W. / Subunit contributions to insect olfactory receptor function : Channel block and odorant recognition. In: Chemical Senses. 2011 ; Vol. 36, No. 9. pp. 781-790.
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